Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free

5 product images

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  Immunoprecipitation - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

  Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619 (purified) at 1/30 immunoprecipitating PPP1CA + 1CB in Jurkat cell lysate. For western blotting, a HRP-conjugated Goat anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1000).

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619).

  All lanes: Immunoprecipitation - Anti-PPP1CA + PPP1CB antibody [EP1511Y] (Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619)

  Predicted band size: 37 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

  Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619 staining PPP1CA + 1CB in the HepG2 cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077(1/500) an Alexa Fluor^®488-conjugated Goat anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619).

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  Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

  Overlay histogram showing HeLa cells stained with Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619 (red line) at 1/150 dilution. The cells were fixed with 80% methanol. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions. Cells also incubated without primary antibody and secondary antibody (blue line)
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

  Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619 staining PP1CA + 1CB in Human cerebrum cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/50). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619).

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  Flow Cytometry (Intracellular) - Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free (ab239844)

  Overlay histogram showing HeLa cells stained with Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619, unpurified (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PPP1CA + PPP1CB antibody [EP1511Y] ab52619).