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AB154600

Anti-PPP1CB antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal PPP1CB antibody. Suitable for WB, ICC/IF, IHC-P, IHC-Fr and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPP1CB aa 1-300.

View Alternative Names

Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-PPP1CB antibody (AB154600)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP1CB antibody (AB154600)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling PPP1CB protein at cytoplasm and nucleus using ab154600 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody (AB154600)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody (AB154600)

Immunohistochemical analysis of paraffin-embedded rat testis tissue stained for PPP1CB protein at cytoplasm and nucleus with ab154600 at a 1/3000 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Frozen sections) - Anti-PPP1CB antibody (AB154600)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-PPP1CB antibody (AB154600)

Immunohistochemical analysis of Frozen sectioned adult mouse cerebellum staining PPP1CB with ab154600 at a dilution of 1/250 (Green). NF-H, stained by NF-H antibody diluted at 1/500 dilution (Red). The nuclear staining is DAPI (Blue).
Antigen Retrieval : Citrate buffer, pH 6.0, 10 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody (AB154600)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody (AB154600)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for PPP1CB protein at cytoplasm and nucleus with ab154600 at a 1/3000 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Western blot - Anti-PPP1CB antibody (AB154600)
  • WB

Supplier Data

Western blot - Anti-PPP1CB antibody (AB154600)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-PPP1CB antibody (ab154600) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

PPP1CB shRNA transfected 293T whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 37 kDa

false

Western blot - Anti-PPP1CB antibody (AB154600)
  • WB

Unknown

Western blot - Anti-PPP1CB antibody (AB154600)

10% SDS PAGE

All lanes:

Western blot - Anti-PPP1CB antibody (ab154600) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Human PPP1CB aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P62140

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

Keep as concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1CB also known as Protein Phosphatase 1 Catalytic Subunit Beta is a member of the catalytic subunit of Protein Phosphatase 1 (PP1). It has an approximate mass of 37 kDa. PPP1CB is expressed in various tissues throughout the human body including the heart brain and skeletal muscle. This phosphatase is primarily involved in the dephosphorylation of proteins a critical function to regulate cellular processes. As a catalytic subunit PPP1CB mediates various signaling cascades by removing phosphate groups from target proteins.
Biological function summary

PPP1CB plays key roles in cell division signal transduction and muscle contraction. It acts as part of a larger complex interacting with a variety of regulatory proteins and substrates to fine-tune cellular activities. Through dephosphorylation PPP1CB aids in the transition between different phases of cell cycles impacting cellular growth and proliferation. It is also involved in glycogen metabolism where it removes phosphate groups from glycogen phosphorylase ensuring proper energy balance in cells.

Pathways

PPP1CB participates significantly in the TGF-beta signaling and Wnt signaling pathways. It interacts with several proteins such as SMADs in the TGF-beta pathway contributing to the regulation of transcription and cellular responses to growth factors. In the Wnt signaling pathway PPP1CB deactivates key kinase members thereby modulating cell fate decisions and embryonic development. These pathways underline the phosphate turnover that PPP1CB controls impacting various cellular functions.

PPP1CB has been associated with cancer development and cardiovascular diseases. It contributes to oncogenesis when its regulatory role in cell cycle controls is disrupted leading to unchecked cell proliferation. Alterations in PPP1CB activity have also been linked to heart diseases where improper dephosphorylation affects muscle contraction and cardiac function. In cancer interactions with proteins such as PP2A alter the signaling balance while in heart disease proteins like PLN may connect to aberrant PPP1CB function emphasizing its critical role in maintaining cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed : 23396208). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed : 35768504, PubMed : 35831509, PubMed : 36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed : 35768504, PubMed : 36175670).
See full target information PPP1CB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 15:506-22 PubMed26598648

2015

Proteomic Analysis of Dynein-Interacting Proteins in Amyotrophic Lateral Sclerosis Synaptosomes Reveals Alterations in the RNA-Binding Protein Staufen1.

Applications

ICC/IF

Species

Unspecified reactive species

Noga Gershoni-Emek,Arnon Mazza,Michael Chein,Tal Gradus-Pery,Xin Xiang,Ka Wan Li,Roded Sharan,Eran Perlson
View all publications
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