Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)

Rabbit Recombinant Monoclonal PPP1CB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (AB284701), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	IP	WB	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Tested	Tested	Expected
Rat	Expected	Expected	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

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Target data

See full target information Serine/threonine-protein phosphatase PP1-beta catalytic subunit

Function

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (PubMed:35768504, PubMed:35831509, PubMed:36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed:35768504, PubMed:35831509, PubMed:36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed:35768504, PubMed:36175670).

Alternative names

Serine/threonine-protein phosphatase PP1-beta catalytic subunit, PP-1B, PPP1CD, PPP1CB

Recommended products

Rabbit Recombinant Monoclonal PPP1CB antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EP1804Y
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab284701 is the carrier-free version of Anti-PPP1CB antibody [EP1804Y] ab53315

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PPP1CB also known as Protein Phosphatase 1 Catalytic Subunit Beta is a member of the catalytic subunit of Protein Phosphatase 1 (PP1). It has an approximate mass of 37 kDa. PPP1CB is expressed in various tissues throughout the human body including the heart brain and skeletal muscle. This phosphatase is primarily involved in the dephosphorylation of proteins a critical function to regulate cellular processes. As a catalytic subunit PPP1CB mediates various signaling cascades by removing phosphate groups from target proteins.

Biological function summary

PPP1CB plays key roles in cell division signal transduction and muscle contraction. It acts as part of a larger complex interacting with a variety of regulatory proteins and substrates to fine-tune cellular activities. Through dephosphorylation PPP1CB aids in the transition between different phases of cell cycles impacting cellular growth and proliferation. It is also involved in glycogen metabolism where it removes phosphate groups from glycogen phosphorylase ensuring proper energy balance in cells.

Pathways

PPP1CB participates significantly in the TGF-beta signaling and Wnt signaling pathways. It interacts with several proteins such as SMADs in the TGF-beta pathway contributing to the regulation of transcription and cellular responses to growth factors. In the Wnt signaling pathway PPP1CB deactivates key kinase members thereby modulating cell fate decisions and embryonic development. These pathways underline the phosphate turnover that PPP1CB controls impacting various cellular functions.

Associated diseases and disorders

PPP1CB has been associated with cancer development and cardiovascular diseases. It contributes to oncogenesis when its regulatory role in cell cycle controls is disrupted leading to unchecked cell proliferation. Alterations in PPP1CB activity have also been linked to heart diseases where improper dephosphorylation affects muscle contraction and cardiac function. In cancer interactions with proteins such as PP2A alter the signaling balance while in heart disease proteins like PLN may connect to aberrant PPP1CB function emphasizing its critical role in maintaining cellular health.

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13 product images

Immunoprecipitation - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Anti-PPP1CB antibody [EP1804Y] ab53315 (purified) at 1/100 dilution (2ug) immunoprecipitating Protein phosphatase 1 beta in Human fetal brain lysate.

Lane 1 (input): Human fetal brain lysate 10ug

Lane 2 (+): Anti-PPP1CB antibody [EP1804Y] ab53315 & Human fetal brain lysate

Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PPP1CB antibody [EP1804Y] ab53315 in Human fetal brain lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1000 dilution.

Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-PPP1CB antibody [EP1804Y] (Anti-PPP1CB antibody [EP1804Y] ab53315)
Predicted band size: 37 kDa
Observed band size: 38 kDa
Western blot - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-PPP1CB antibody [EP1804Y] (Anti-PPP1CB antibody [EP1804Y] ab53315) at 1/50000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
Lane 2: Mouse brain lysates at 20 µg
Lane 3: Rat brain lysates at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical carcinoma tissue sections labeling PPP1CB with purified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Flow Cytometry (Intracellular) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PPP1CB with purified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/40 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - 90% methanol. Unlabeled control - Rabbit monoclonal IgG (Black). Cell without incubation with primary antibody and secondary antibody (Blue).
Immunocytochemistry/ Immunofluorescence - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Unpurified Anti-PPP1CB antibody [EP1804Y] ab53315 staining PPP1CB antibody in HeLa (human cervix adenocarcinoma) cells by ICC (Immunocytochemistry). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney tissue sections labeling PPP1CB with purified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling PPP1CB with purified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/400 dilution (1 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH 9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PPP1CB antibody [EP1804Y] (Anti-PPP1CB antibody [EP1804Y] ab53315) at 1/20000 dilution
All lanes: Jurkat cells at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Predicted band size: 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of MCF-7 (Human breast adenocarcinoma epithelial cell) cells labeling PPP1CB with Purified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/250 dilution (1.6μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of human cervix carcinoma labelling PPP1CB with unpurified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of mouse cardiac muscle labelling PPP1CB with unpurified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded sections of rat colon labelling PPP1CB with unpurified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/250 dilution. Prediluted ImmunoHistoprobe (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Counter stain = Hematoxylin. Heat mediated antigen retrieval using EDTA buffer at pH 9 was performed.
Flow Cytometry (Intracellular) - Anti-PPP1CB antibody [EP1804Y] - BSA and Azide free (ab284701)
This data was developed using Anti-PPP1CB antibody [EP1804Y] ab53315, the same antibody clone in a different buffer formulation.
Flow Cytometry (Intracellular) analysis of HeLa cells labelling PPP1CB with unpurified Anti-PPP1CB antibody [EP1804Y] ab53315 at 1/20 dilution. Goat anti rabbit IgG (H&L) (FITC) at 1/150 dilution was used as the secondary antibody. Isotype control = Rabbit monoclonal IgG. Fixative = 2% paraformaldehyde.