AB177043

Anti-PPP1R11 antibody

Name: Anti-PPP1R11 antibody (ab177043) | Abcam
Brand: Abcam Limited
SKU: AB177043
Rating: 3 (2 reviews)

(2 Reviews)

(1 Publication)

Rabbit Polyclonal PPP1R11 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPP1R11 aa 1 to C-terminus.

View Alternative Names

HCGV, TCTE5, PPP1R11, E3 ubiquitin-protein ligase PPP1R11, Hemochromatosis candidate gene V protein, Protein phosphatase 1 regulatory subunit 11, Protein phosphatase inhibitor 3, HCG V

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R11 antibody (AB177043)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R11 antibody (AB177043)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling PPP1R11 with ab177043 at 1/100 dilution.

Supplier Data

Western blot - Anti-PPP1R11 antibody (AB177043)

All lanes:

Western blot - Anti-PPP1R11 antibody (ab177043) at 1/1000 dilution

All lanes:

HepG2 cell lysate

Predicted band size: 14 kDa

Observed band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PPP1R11 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60927

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1R11 also known as Protein Phosphatase 1 Regulatory Inhibitor Subunit 11 is a protein that acts as an inhibitor of protein phosphatase 1 (PP1) activity. It weighs approximately 14 kDa and is expressed in various tissues with higher levels in the testis and less in other tissues. Its primary role involves binding to PP1 a critical enzyme in the regulation of phosphorylation states within cells and modulating its activity.

Biological function summary

The protein regulates the phosphorylation process which is vital for cellular functions. PPP1R11 does not work in isolation; it forms part of a complex with PP1 influencing the enzyme's ability to dephosphorylate target substrates. This function is essential in controlling cell division protein synthesis and muscle contractility through its inhibition effect on PP1.

Pathways

PPP1R11 contributes significantly to cellular signaling pathways. It plays a role in the cell cycle regulation pathway and also the glycogen metabolism pathway. Within these pathways it interacts with PP1 and other regulatory proteins to maintain proper cellular functions and energy management. Alterations in these interactions can lead to dysregulation exhibiting the target's importance in cell physiology.

The alterations in the function or expression of PPP1R11 link to certain cancers and metabolic conditions. Its connection with PP1 shows a relationship with cancer through irregular cell cycle control. Furthermore its role in glycogen metabolism can relate to disorders like type 2 diabetes highlighting the interaction with insulin-regulatory proteins. Understanding PPP1R11’s function can help develop potential interventions for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Atypical E3 ubiquitin-protein ligase which ubiquitinates TLR2 at 'Lys-754' leading to its degradation by the proteasome. Plays a role in regulating inflammatory cytokine release and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2 (PubMed : 27805901). Inhibitor of protein phosphatase 1 (PubMed : 9843442).

See full target information PPP1R11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal for immunotherapy of cancer 8: PubMed32188703

2020

Simultaneous inhibition of CXCR1/2, TGF-β, and PD-L1 remodels the tumor and its microenvironment to drive antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas A Horn,Jeffrey Riskin,Heidi A Hempel,Kristen Fousek,Hanne Lind,Duane H Hamilton,Kristen K McCampbell,Dean Y Maeda,John A Zebala,Zhen Su,Jeffrey Schlom,Claudia Palena

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP1R11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R11 antibody (AB177043)

Western blot - Anti-PPP1R11 antibody (AB177043)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise