AB171960

Anti-PPP1R11 antibody [EPR11965]

Name: Anti-PPP1R11 antibody [EPR11965] (ab171960) | Abcam
Brand: Abcam Limited
SKU: AB171960
Price: 650 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal PPP1R11 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

HCGV, TCTE5, PPP1R11, E3 ubiquitin-protein ligase PPP1R11, Hemochromatosis candidate gene V protein, Protein phosphatase 1 regulatory subunit 11, Protein phosphatase inhibitor 3, HCG V

3 Images

Flow Cytometry (Intracellular) - Anti-PPP1R11 antibody [EPR11965] (AB171960)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPP1R11 antibody [EPR11965] (AB171960)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling PPP1R11 with ab171960 at 1/10 dilution (red)or a Rabbit IgG (negative) (green).

Supplier Data

Immunoprecipitation - Anti-PPP1R11 antibody [EPR11965] (AB171960)

Western blot analysis on immunoprecipitation pellet from Human testis lysate (1) labeling PPP1R11 with ab171960 at 1/10 dilution or 1X PBS (2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

All lanes:

Immunoprecipitation - Anti-PPP1R11 antibody [EPR11965] (ab171960)

Predicted band size: 14 kDa

false

Supplier Data

Western blot - Anti-PPP1R11 antibody [EPR11965] (AB171960)

All lanes:

Western blot - Anti-PPP1R11 antibody [EPR11965] (ab171960) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Fetal brain tissue lysate at 10 µg

Lane 4:

NIH:OVCAR-3 cell lysate at 10 µg

Lane 5:

MDA-MB-435 lysate at 10 µg

Predicted band size: 14 kDa

false

Carrier free

Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11965

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1R11 also known as Protein Phosphatase 1 Regulatory Inhibitor Subunit 11 is a protein that acts as an inhibitor of protein phosphatase 1 (PP1) activity. It weighs approximately 14 kDa and is expressed in various tissues with higher levels in the testis and less in other tissues. Its primary role involves binding to PP1 a critical enzyme in the regulation of phosphorylation states within cells and modulating its activity.

Biological function summary

The protein regulates the phosphorylation process which is vital for cellular functions. PPP1R11 does not work in isolation; it forms part of a complex with PP1 influencing the enzyme's ability to dephosphorylate target substrates. This function is essential in controlling cell division protein synthesis and muscle contractility through its inhibition effect on PP1.

Pathways

PPP1R11 contributes significantly to cellular signaling pathways. It plays a role in the cell cycle regulation pathway and also the glycogen metabolism pathway. Within these pathways it interacts with PP1 and other regulatory proteins to maintain proper cellular functions and energy management. Alterations in these interactions can lead to dysregulation exhibiting the target's importance in cell physiology.

The alterations in the function or expression of PPP1R11 link to certain cancers and metabolic conditions. Its connection with PP1 shows a relationship with cancer through irregular cell cycle control. Furthermore its role in glycogen metabolism can relate to disorders like type 2 diabetes highlighting the interaction with insulin-regulatory proteins. Understanding PPP1R11’s function can help develop potential interventions for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Atypical E3 ubiquitin-protein ligase which ubiquitinates TLR2 at 'Lys-754' leading to its degradation by the proteasome. Plays a role in regulating inflammatory cytokine release and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2 (PubMed : 27805901). Inhibitor of protein phosphatase 1 (PubMed : 9843442).

See full target information PPP1R11

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1543 PubMed39934163

2025

BBOX1 restrains TBK1-mTORC1 oncogenic signaling in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chengheng Liao,Lianxin Hu,Liwei Jia,Jin Zhou,Tao Wang,Kangsan Kim,Hua Zhong,Hongwei Yao,Lei Dong,Lei Guo,Qian Liang,Cheng Zhang,Fangzhou Zhao,Jun Fang,Hongyi Liu,Shina Li,Lin Xu,Jeremy M Simon,Srinivas Malladi,Payal Kapur,James Brugarolas,Ralph J DeBerardinis,Qing Zhang

The Journal of clinical investigation 133: PubMed37022795

2023

Long noncoding RNA HIKER regulates erythropoiesis in Monge's disease via CSNK2B.

Applications

Unspecified application

Species

Unspecified reactive species

Priti Azad,Dan Zhou,Hung-Chi Tu,Francisco C Villafuerte,David Traver,Tariq M Rana,Gabriel G Haddad

The Journal of clinical investigation 132: PubMed35362478

2022

Tankyrase represses autoinflammation through the attenuation of TLR2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Matsumoto,Ioannis D Dimitriou,Jose La Rose,Melissa Lim,Susan Camilleri,Napoleon Law,Hibret A Adissu,Jiefei Tong,Michael F Moran,Andrzej Chruscinski,Fang He,Yosuke Asano,Takayuki Katsuyama,Ken-Ei Sada,Jun Wada,Robert Rottapel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP1R11 antibody [EPR11965]

Flow Cytometry (Intracellular) - Anti-PPP1R11 antibody [EPR11965] (AB171960)

Immunoprecipitation - Anti-PPP1R11 antibody [EPR11965] (AB171960)

Western blot - Anti-PPP1R11 antibody [EPR11965] (AB171960)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise