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AB249675

Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPP1R11 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

HCGV, TCTE5, PPP1R11, E3 ubiquitin-protein ligase PPP1R11, Hemochromatosis candidate gene V protein, Protein phosphatase 1 regulatory subunit 11, Protein phosphatase inhibitor 3, HCG V

3 Images
Flow Cytometry (Intracellular) - Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free (AB249675)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free (AB249675)

This data was developed using ab171960, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling PPP1R11 with ab171960 at 1/10 dilution (red) or a Rabbit IgG (negative) (green).

Immunoprecipitation - Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free (AB249675)
  • IP

Supplier Data

Immunoprecipitation - Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free (AB249675)

This data was developed using ab171960, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Human testis lysate (1) labeling PPP1R11 with ab171960 at 1/10 dilution or 1X PBS (2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

All lanes:

Immunoprecipitation - Anti-PPP1R11 antibody [EPR11965] (<a href='/en-us/products/primary-antibodies/ppp1r11-antibody-epr11965-ab171960'>ab171960</a>)

Predicted band size: 14 kDa

false

Western blot - Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free (AB249675)
  • WB

Supplier Data

Western blot - Anti-PPP1R11 antibody [EPR11965] - BSA and Azide free (AB249675)

This data was developed using ab171960, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPP1R11 antibody [EPR11965] (<a href='/en-us/products/primary-antibodies/ppp1r11-antibody-epr11965-ab171960'>ab171960</a>) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Fetal brain tissue lysate at 10 µg

Lane 4:

NIH:OVCAR-3 cell lysate at 10 µg

Lane 5:

MDA-MB-435 lysate at 10 µg

Predicted band size: 14 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11965

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249675 is the carrier-free version of ab171960.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1R11 also known as Protein Phosphatase 1 Regulatory Inhibitor Subunit 11 is a protein that acts as an inhibitor of protein phosphatase 1 (PP1) activity. It weighs approximately 14 kDa and is expressed in various tissues with higher levels in the testis and less in other tissues. Its primary role involves binding to PP1 a critical enzyme in the regulation of phosphorylation states within cells and modulating its activity.
Biological function summary

The protein regulates the phosphorylation process which is vital for cellular functions. PPP1R11 does not work in isolation; it forms part of a complex with PP1 influencing the enzyme's ability to dephosphorylate target substrates. This function is essential in controlling cell division protein synthesis and muscle contractility through its inhibition effect on PP1.

Pathways

PPP1R11 contributes significantly to cellular signaling pathways. It plays a role in the cell cycle regulation pathway and also the glycogen metabolism pathway. Within these pathways it interacts with PP1 and other regulatory proteins to maintain proper cellular functions and energy management. Alterations in these interactions can lead to dysregulation exhibiting the target's importance in cell physiology.

The alterations in the function or expression of PPP1R11 link to certain cancers and metabolic conditions. Its connection with PP1 shows a relationship with cancer through irregular cell cycle control. Furthermore its role in glycogen metabolism can relate to disorders like type 2 diabetes highlighting the interaction with insulin-regulatory proteins. Understanding PPP1R11’s function can help develop potential interventions for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Atypical E3 ubiquitin-protein ligase which ubiquitinates TLR2 at 'Lys-754' leading to its degradation by the proteasome. Plays a role in regulating inflammatory cytokine release and gram-positive bacterial clearance by functioning, in part, through the ubiquitination and degradation of TLR2 (PubMed : 27805901). Inhibitor of protein phosphatase 1 (PubMed : 9843442).
See full target information PPP1R11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com