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AB246012

Anti-PPP1R14B/PNG antibody

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(1 Publication)

Rabbit Polyclonal PPP1R14B/PNG antibody. Suitable for IP and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP1R14B aa 1-100.

View Alternative Names

PLCB3N, PNG, PPP1R14B, Protein phosphatase 1 regulatory subunit 14B, Phospholipase C-beta-3 neighbouring gene protein

1 Images
Immunoprecipitation - Anti-PPP1R14B/PNG antibody (AB246012)
  • IP

Supplier Data

Immunoprecipitation - Anti-PPP1R14B/PNG antibody (AB246012)

PPP1R14B/PNG was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab246012 at 10 μg/mg lysate. Western blot was performed from the immunoprecipitate using an alternative anti-PPP1R14B/PNG antibody, which recognizes a downstream epitope at 1 μg/ml.

Lane 1 : ab246012 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PPP1R14B/PNG antibody (ab246012)

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human PPP1R14B aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q96C90

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-10 µg/mg of lysate", "IP-species-notes": "<p></p>" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab246012 was affinity purified using an epitope specific to PPP1R14B/PNG immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1R14B also known as protein phosphatase 1 regulatory inhibitor subunit 14B or PNG is an important inhibitor of protein phosphatase 1 (PP1). This inhibitor modulates PP1 activity by binding to it resulting in inhibition of its phosphatase activity. The protein has a molecular mass of approximately 17 kDa. PPP1R14B is widely expressed in human tissues with specific abundance in smooth muscle and the brain indicating its role in functions related to these tissues.
Biological function summary

PPP1R14B plays a role in smooth muscle contraction regulation and neuronal signaling. By acting as a regulatory subunit it takes part in several complexes that modulate phosphatase activity important to muscle contraction and neurotransmitter release processes. The inhibition of PP1 by PPP1R14B modulates the phosphorylation status of its substrates which affects the strength and duration of muscle cell contractions and neuronal response.

Pathways

PPP1R14B integrates into signaling cascades such as the cAMP-dependent signaling pathway and the RhoA/ROCK pathway. It influences the regulation of muscular and neuronal excitability by impacting proteins like myosin light chain (MLC) and synapsins which are downstream targets in these pathways. Interaction with these pathways ensures precise control over muscular contraction and synaptic transmission facilitating proper cellular responses to external stimuli.

PPP1R14B has relevance in conditions like hypertension and neurological disorders which involve dysregulated smooth muscle and neuronal activity. In hypertension its interaction with MLC may affect blood vessel tension. In neurological conditions interactions with synapsins can affect neuronal communication and plasticity. Understanding its role and regulation offers insights into potential therapeutic targets for disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibitor of PPP1CA. Has over 50-fold higher inhibitory activity when phosphorylated (By similarity).
See full target information PPP1R14B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:1989-2005 PubMed38088504

2023

OTU deubiquitinase 7B facilitates the hyperthermia-induced inhibition of lung cancer progression through enhancing Smac-mediated mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Sun,Jun Bai,Jingying Sun,Yang Sun,Fan Zhang,He Li,Ying Liu,Lian Meng,Xifang Wang
View all publications
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Product promise

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