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AB76785

Anti-PPP1R15B antibody

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(1 Publication)

Rabbit Polyclonal PPP1R15B antibody. Suitable for WB, IHC-Fr and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP1R15B.

View Alternative Names

Protein phosphatase 1 regulatory subunit 15B, PPP1R15B

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-Fr

applications

Immunogen

Synthetic Peptide within Human PPP1R15B. The exact immunogen used to generate this antibody is proprietary information.

Q5SWA1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1R15B also known as GADD34 is a regulatory subunit of protein phosphatase 1 (PP1). It functions mechanically by associating with PP1 to regulate the dephosphorylation of the eukaryotic initiation factor 2 alpha (eIF2α). This interaction is essential for controlling protein synthesis especially during stress conditions. The molecular mass of PPP1R15B is approximately 74 kDa. Expression of PPP1R15B occurs in various tissues with high levels in brain liver and kidney tissues.
Biological function summary

The regulatory action of PPP1R15B on eIF2α impacts protein synthesis by reversing the phosphorylation which occurs in response to cellular stress. It functions as part of a multi-protein complex that includes PP1 and possibly other proteins involved in cellular stress responses. As a result it helps in the recovery of protein translation during periods of endoplasmic reticulum stress contributing to cell survival pathways under transient stress conditions.

Pathways

PPP1R15B plays a significant role in the integrated stress response pathway by modulating protein synthesis. It participates in the unfolded protein response (UPR) which is important when cells encounter endoplasmic reticulum stress. Through its interaction with eIF2α and other associated components like PP1 PPP1R15B facilitates cellular adaptation to changing environmental stressors.

PPP1R15B has been implicated in diseases such as neurodegenerative disorders and certain types of cancer. Deregulation of its function can lead to aberrant protein synthesis control which results in pathological outcomes. In neurodegenerative diseases its interaction with proteins like eIF2α is significant affecting neuronal cell survival under stress. In cancer altered expression or function of PPP1R15B may contribute to tumor progression due to its role in managing cellular stress responses and apoptosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Maintains low levels of EIF2S1 phosphorylation in unstressed cells by promoting its dephosphorylation by PP1.
See full target information PPP1R15B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed36077620

2022

Temsirolimus Enhances Anti-Cancer Immunity by Inducing Autophagy-Mediated Degradation of the Secretion of Small Extracellular Vesicle PD-L1.

Applications

Unspecified application

Species

Unspecified reactive species

Seong-Sik Park,Jong-In Kim,Chan-Hyeong Lee,Ju-Hyun Bae,Ju-Mi Park,Eun-Ji Choe,Moon-Chang Baek
View all publications
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Product promise

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