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AB89135

Anti-PPP1R16A antibody

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(1 Publication)

Mouse Polyclonal PPP1R16A antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PPP1R16A.

View Alternative Names

MYPT3, PPP1R16A, Protein phosphatase 1 regulatory subunit 16A, Myosin phosphatase-targeting subunit 3

2 Images
Western blot - Anti-PPP1R16A antibody (AB89135)
  • WB

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Western blot - Anti-PPP1R16A antibody (AB89135)

All lanes:

Western blot - Anti-PPP1R16A antibody (ab89135) at 1 µg/mL

All lanes:

Human pancreas lysate at 50 µg

Predicted band size: 58 kDa

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Western blot - Anti-PPP1R16A antibody (AB89135)
  • WB

Unknown

Western blot - Anti-PPP1R16A antibody (AB89135)

All lanes:

Western blot - Anti-PPP1R16A antibody (ab89135) at 1 µg/mL

Lane 1:

PPP1R16A-transfected 293T cell lysate at 25 µg

Lane 2:

non transfected lysate at 25 µg

Predicted band size: 58 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PPP1R16A.

Q96I34

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PPP1R16A protein also known as myosin light chain phosphatase target subunit 1 is part of the protein phosphatase 1 regulatory subunits. It weighs approximately 84 kDa and is mainly found in tissues like brain and skeletal muscle. It functions as a regulatory subunit modulating the activity of protein phosphatase 1 (PP1) a critical enzyme in cellular signaling.
Biological function summary

This protein plays a significant role in the regulation of myosin phosphatase activity influencing muscle function. As part of a multi-subunit complex PPP1R16A interacts with PP1 to dephosphorylate myosin light chains which is essential for muscle relaxation. This process ensures proper muscle contraction and relaxation cycles in response to cellular signals.

Pathways

Protein phosphatase activity plays key roles in both the smooth muscle contraction and Rho GTPase signaling pathways. PPP1R16A by regulating myosin light chain phosphorylation status interacts with proteins like Rho-associated coiled-coil containing protein kinase (ROCK) within these pathways. This involvement positions PPP1R16A as a vital player in pathways that govern cell motility and muscle contraction.

The dysregulation of PPP1R16A activity is associated with conditions such as hypertension and asthma. Its interaction with ROCK and misregulation in myosin phosphatase activity can contribute to abnormal muscle contractility playing a role in the pathophysiology of these diseases. Understanding this interaction helps in exploring therapeutic targets for related cardiovascular and respiratory disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits protein phosphatase 1 activity toward phosphorylase, myosin light chain and myosin substrates.
See full target information PPP1R16A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 41:101874 PubMed38262113

2024

High expression of B7-H3 on monocyte/macrophages in tumor microenvironment promotes lung cancer progression by inhibiting apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongze Zhang,Haitao Huang,Xin Gao,Gehua Yu,Xueguang Zhang,Haiyan Jin,Ruyan Xu,Zhenxin Wang,Guangbo Zhang
View all publications
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