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AB235049

Anti-PPP1R3B antibody

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(1 Publication)

Rabbit Polyclonal PPP1R3B antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PPP1R3B.

View Alternative Names

PPP1R4, PPP1R3B, Protein phosphatase 1 regulatory subunit 3B, Hepatic glycogen-targeting protein phosphatase 1 regulatory subunit GL, Protein phosphatase 1 regulatory subunit 4, Protein phosphatase 1 subunit GL, PP1 subunit R4, PTG

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3B antibody (AB235049)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3B antibody (AB235049)

Paraffin-embedded human skeletal muscle tissue stained for PPP1R3B using ab235049 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3B antibody (AB235049)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3B antibody (AB235049)

Paraffin-embedded human liver tissue stained for PPP1R3B using ab235049 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-PPP1R3B antibody (AB235049)
  • WB

Supplier Data

Western blot - Anti-PPP1R3B antibody (AB235049)

All lanes:

Western blot - Anti-PPP1R3B antibody (ab235049) at 1/500 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit at 1/10000 dilution

Predicted band size: 33 kDa

false

Western blot - Anti-PPP1R3B antibody (AB235049)
  • WB

Supplier Data

Western blot - Anti-PPP1R3B antibody (AB235049)

All lanes:

Western blot - Anti-PPP1R3B antibody (ab235049) at 1/500 dilution

All lanes:

Rat heart tissue lysate

Secondary

All lanes:

Goat polyclonal to rabbit at 1/50000 dilution

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PPP1R3B.

Q86XI6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP1R3B also known as Protein Phosphatase 1 Regulatory Subunit 3B plays a mechanical role in glycogen metabolism. The protein affects the activity of glycogen synthase and glycogen phosphorylase by regulating the substrate specificity of Protein Phosphatase 1 (PP1) its enzymatic partner. PPP1R3B has a mass of approximately 33 kDa and its expression is primarily noted in liver and skeletal muscle tissues. The localization of PPP1R3B to these tissues suggests a specialized role in energy storage and regulation.
Biological function summary

Protein Phosphatase 1 Regulatory Subunit 3B influences glycogen synthesis and degradation. As a part of the PP1 holoenzyme complex PPP1R3B modulates the dephosphorylation of target proteins which controls glycogen metabolism. This regulation enables cells to respond to changes in energy demands effectively. PPP1R3B's interaction with other subunits in the PP1 complex allows for dynamic control of the metabolic processes impacting overall energy homeostasis.

Pathways

Regulation of glycogen metabolism involves several important steps where PPP1R3B integrates into the glycogen biosynthetic pathway and the insulin signaling pathway. By forming associations with other proteins such as glycogen synthase it exerts influence on how glycogen is synthesized and mobilized. In response to signals from upstream kinases such as Akt PPP1R3B works with PP1 to ensure the precise modulation of phosphorylation states that dictate glycogen synthase activity.

Irregularities in PPP1R3B functionality connect to conditions like type 2 diabetes and glycogen storage disease. Disruptions in its normal operation can result in improper glycogen mobilization and storage influencing blood glucose levels. The protein interacts with other regulatory proteins like the PP1 complex and AMPK which are involved in pathways linked to insulin sensitivity and energy balance. Understanding PPP1R3B's role in these disorders could provide insights for therapeutic strategies to manage metabolic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a glycogen-targeting subunit for phosphatase PP1. Facilitates interaction of the PP1 with enzymes of the glycogen metabolism and regulates its activity. Suppresses the rate at which PP1 dephosphorylates (inactivates) glycogen phosphorylase and enhances the rate at which it activates glycogen synthase and therefore limits glycogen breakdown. Its activity is inhibited by PYGL, resulting in inhibition of the glycogen synthase and glycogen phosphorylase phosphatase activities of PP1. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in hepatocytes (By similarity).
See full target information PPP1R3B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 221: PubMed34747980

2021

Ubiquitin ligase MARCH5 localizes to peroxisomes to regulate pexophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zheng,Xi Chen,Qiang Liu,Guisheng Zhong,Min Zhuang
View all publications
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