Rabbit Polyclonal PPP1R3C antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP1R3C aa 1-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PPP1R3C aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQK1
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/50.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Target data
Function
Acts as a glycogen-targeting subunit for PP1 and regulates its activity. Activates glycogen synthase, reduces glycogen phosphorylase activity and limits glycogen breakdown. Dramatically increases basal and insulin-stimulated glycogen synthesis upon overexpression in a variety of cell types.
Alternative names
PPP1R5, PPP1R3C, Protein phosphatase 1 regulatory subunit 3C, Protein phosphatase 1 regulatory subunit 5, Protein targeting to glycogen, PP1 subunit R5, PTG
Recommended products
Rabbit Polyclonal PPP1R3C antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP1R3C aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PPP1R3C aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UQK1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PPP1R3C also known as protein phosphatase 1 regulatory subunit 3C is a regulatory subunit of protein phosphatase 1 (PP1). This protein has a mass of approximately 34 kDa. PPP1R3C is mainly expressed in skeletal muscle and liver tissues. It acts by modulating glycogen metabolism where it impacts glycogen synthesis and breakdown. This regulation is achieved through altering the activity of PP1 which dephosphorylates diverse biological substrates to control various cellular functions.
- Biological function summary
The PPP1R3C protein binds to glycogen and PP1 forming a complex with these substrates. This interaction plays a role in the regulation of glucose homeostasis by controlling glycogen synthesis and breakdown. Such processes are important for energy storage and release in muscle and liver tissues. By integrating within this complex PPP1R3C influences the activity and localization of PP1 facilitating efficient glycogen regulation.
- Pathways
PPP1R3C participates in critical pathways like glycogen metabolism and insulin signaling. The protein is closely associated with key metabolic proteins such as glycogen synthase. Within these pathways PPP1R3C modifies the enzymatic activity of its pathway partners to balance glucose levels in response to insulin. This involvement supports metabolic flexibility and energy management in response to dietary changes.
- Associated diseases and disorders
PPP1R3C connects to insulin resistance and type 2 diabetes where its altered function may disrupt normal glycogen metabolism. The protein's dysfunction impacts glucose homeostasis and insulin signaling pathway contributing to the pathophysiology of these metabolic diseases. Additionally PPP1R3C interacts with proteins like glycogen synthase which play significant roles in the metabolic disturbances seen in such disorders. Understanding these interactions helps in devising strategies for targeted therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-PPP1R3C antibody (ab251771)
Western blot analysis of RT-4 cell lysate labeling PPP1R3C with ab251771.
All lanes: Western blot - Anti-PPP1R3C antibody (ab251771) at 0.04 µg/mL
All lanes: RT-4 cell lysate
Predicted band size: 36 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3C antibody (ab251771)
Formalin-fixed, paraffin-embedded human liver tissue stained for PP1R3C with ab251771 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3C antibody (ab251771)
Formalin-fixed, paraffin-embedded human lung tissue stained for PP1R3C with ab251771 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3C antibody (ab251771)
Formalin-fixed, paraffin-embedded human heart tissue stained for PP1R3C with ab251771 at a 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP1R3C antibody (ab251771)
Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for PP1R3C with ab251771 at a 1/50 dilution in immunohistochemical analysis.
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