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AB249465

Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal PP2AB antibody. Carrier free. Suitable for WB and reacts with Human, Mouse samples.

View Alternative Names

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, PP2A-beta, PPP2CB

2 Images
Western blot - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (AB249465)
  • WB

Lab

Western blot - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (AB249465)

This data was developed using ab168371, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 whole cell lysate (20 Âμg)

Lane 2 : PPP2CB knockout HAP1 whole cell lysate (20 Âμg)

Lane 3 : HeLa whole cell lysate (20 Âμg)

Lane 4 : A431 whole cell lysate (20 Âμg)

ab168371 was shown to specifically react with PPP2CB in wild-type HAP1 cells. No band was observed when PPP2CB knockout samples were examined. Wild-type and PPP2CB knockout samples were subjected to SDS-PAGE. ab168371 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (<a href='/en-us/products/primary-antibodies/ppp2cb-antibody-epr11786b-ab168371'>ab168371</a>)

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Western blot - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (AB249465)
  • WB

Unknown

Western blot - Anti-PPP2CB antibody [EPR11786(B)] - BSA and Azide free (AB249465)

This data was developed using ab168371, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPP2CB antibody [EPR11786(B)] (<a href='/en-us/products/primary-antibodies/ppp2cb-antibody-epr11786b-ab168371'>ab168371</a>) at 1/10000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

HeLa lysate at 10 µg

Lane 3:

Human fetal brain lysate at 10 µg

Lane 4:

Human fetal heart lysate at 10 µg

Lane 5:

NIH3T3 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11786(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249465 is the carrier-free version of ab168371.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP2CB also known as protein phosphatase 2 catalytic subunit beta is an enzyme that plays an important role in cellular functions. It plays a catalytic role in dephosphorylating serine and threonine residues in various proteins. The alternative names used for PPP2CB include PP2Acβ and PP2A(C subunit)β. This protein has a mass of approximately 35 kDa and is expressed in a variety of tissues with significant presence in heart skeletal muscle and brain. Researchers note its expression in both cytoplasmic and nuclear locations within cells.
Biological function summary

The protein phosphatase activity of PPP2CB influences many cellular processes. It is a vital part of the protein phosphatase 2 (PP2A) complex which is a major serine/threonine phosphatase in cells. Through its involvement in the PP2A complex PPP2CB regulates cellular processes like cell growth division and apoptosis. The PP2A complex of which PPP2CB is a component remains essential to signaling pathways contributing to the dynamic behavior of cellular phosphorylation-dephosphorylation cycles.

Pathways

Protein phosphatase 2's function aligns PP2CB with key signaling routes such as the MAPK and Wnt pathways. In the MAPK pathway PP2A complex dephosphorylates specific kinases which controls cellular responses to external stimuli. Within the Wnt pathway the regulatory actions of PP2A maintain the appropriate level of pathway activity. The roles of PPP2CB in these pathways connect it to other related proteins like B56 family regulatory subunits in signal transduction networks.

Disruptions in PPP2CB activity link to several conditions including cancer and neurodegenerative diseases. The dysregulation of PPP2CB in cancer is often associated with altered cell proliferation and survival as PP2A is a tumor suppressor complex. In neurodegenerative diseases particularly Alzheimer's aberrant PPP2CB activity disrupts phosphorylation states of tau protein contributing to disease pathogenesis. PPP2CB interacts with other significant proteins like tau in Alzheimer's highlighting its importance in maintaining cellular and neurological health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events (Probable). PP2A can modulate the activity of phosphorylase B kinase, casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753).
See full target information PPP2CB
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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