Anti-PPP2R1A antibody [6G3] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PPP2R1A with ab24736 at 7.85μg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor^® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor^®488 Goat anti-rat secondary at 1/1000 dilution.

This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling PPP2R1A with ab24736 at 7.85μg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor^® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on C6 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor^®488 Goat anti-rat secondary at 1/1000 dilution.

This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PPP2R1A with ab24736 at 7.85μg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor^® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows :
-ve control 1 : ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2 : ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor^®488 Goat anti-rat secondary at 1/1000 dilution.

This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

• WB

Supplier Data

Western blot - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976)

Blocking/Dilution buffer : 5% NFDM/TBST.

This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

All lanes:

Western blot - Anti-PPP2R1A antibody [6G3] (<a href='/en-us/products/primary-antibodies/ppp2r1a-antibody-6g3-ab24736'>ab24736</a>) at 0.785 µg/mL

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 3:

Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 6:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Predicted band size: 65 kDa

false

Exposure time: 3s