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Rat Recombinant Monoclonal PPP2R1A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976), expandable thumbnail
  • Western blot - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (AB255976), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Rat
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IF
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Tested
Rat
Not recommended
Tested
Tested

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.785 µg/mL
Notes

-

Species
Rat
Dilution info
0.785 µg/mL
Notes

-

Species
Human
Dilution info
0.785 µg/mL
Notes

-

Tested
Tested

Species
Mouse
Dilution info
7.85 µg/mL
Notes

-

Species
Rat
Dilution info
7.85 µg/mL
Notes

-

Species
Human
Dilution info
7.85 µg/mL
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753, PubMed:33633399). Regulates the recruitment of the SKA complex to kinetochores (PubMed:28982702).

Alternative names

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PPP2R1A

Recommended products

Rat Recombinant Monoclonal PPP2R1A antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
6G3
Purification technique
Ion exchange chromatography
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab255976 is the carrier-free version of Anti-PPP2R1A antibody [6G3] ab24736.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein phosphatase 2 scaffold subunit A alpha isoform (PPP2R1A) functions as an important regulatory component of the serine/threonine phosphatase PP2A complex. Alternate names for PPP2R1A include PR65A and Aalpha. This protein has a mass of approximately 65 kDa and is widely expressed in many tissues including the brain heart and lungs. It serves as a scaffold for regulatory and catalytic subunits facilitating their assembly into the active enzyme complex.

Biological function summary

The PP2A complex plays a role in cell cycle control apoptosis and signal transduction. PPP2R1A forms a dimer with one catalytic and one regulatory subunit. This arrangement influences various cell signaling processes controlling cell growth and division. Through its position in the PP2A complex PPP2R1A moderates phosphorylation status of target proteins impacting diverse cellular activities.

Pathways

PPP2R1A participates in pathways like the Wnt signaling and the MAPK pathways acting as a brake on kinase-driven signaling. In the Wnt signaling pathway PP2A mediates beta-catenin degradation which influences gene expression related to cell growth. Within the MAPK pathway PPP2R1A interacts with kinases to modulate cellular responses to growth factors. Proteins such as AXIN1 in the Wnt signaling context are associated with the regulatory effects mediated by PPP2R1A.

Associated diseases and disorders

Alterations in PPP2R1A have been implicated in cancers like ovarian and uterine serous carcinoma. Mutations within this gene can disrupt PP2A activity leading to unchecked cell proliferation. Associations with beta-catenin highlight a significant role in tumorigenesis. In these contexts PPP2R1A's relationship with proteins such as beta-catenin and other components of the Wnt pathway indicates its importance in maintaining cellular homeostasis and its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com