Rabbit Polyclonal PPP2R1A antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 300 to C-terminus.
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Publications
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- International journal of molecular sciences 24:2023Advanced Optical Microscopy: Unveiling Functional Insights Regarding a Novel Variant and Its Unreported Phenotype.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMònica Roldán,Gregorio Alexander Nolasco,Lluís Armengol,Marcos Frías,Marta Morell,Manel García-Aragonés,Florencia Epifani,Jordi Muchart,María Luisa Ramírez-Almaraz,Loreto Martorell,Cristina Hernando-Davalillo,Roser Urreizti,Mercedes SerranoPubMed 37762002
- British journal of pharmacology 176:1745-17632019Hypoxia modulates protein phosphatase 2A through HIF-1α dependent and independent mechanisms in human aortic smooth muscle cells and ventricular cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsmail Suliman Elgenaidi et. al.PubMed 30825189
- Science translational medicine 9:2017An RNA interference screen identifies druggable regulators of MeCP2 stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura M Lombardi,Manar Zaghlula,Yehezkel Sztainberg,Steven A Baker,Tiemo J Klisch,Amy A Tang,Eric J Huang,Huda Y ZoghbiPubMed 28835516
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P30153
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig, Xenopus tropicalis | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Pig, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed:15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed:16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed:18782753, PubMed:33633399). Regulates the recruitment of the SKA complex to kinetochores (PubMed:28982702).
Alternative names
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PPP2R1A
Recommended products
Rabbit Polyclonal PPP2R1A antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 300 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P30153
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein phosphatase 2 scaffold subunit A alpha isoform (PPP2R1A) functions as an important regulatory component of the serine/threonine phosphatase PP2A complex. Alternate names for PPP2R1A include PR65A and Aalpha. This protein has a mass of approximately 65 kDa and is widely expressed in many tissues including the brain heart and lungs. It serves as a scaffold for regulatory and catalytic subunits facilitating their assembly into the active enzyme complex.
- Biological function summary
The PP2A complex plays a role in cell cycle control apoptosis and signal transduction. PPP2R1A forms a dimer with one catalytic and one regulatory subunit. This arrangement influences various cell signaling processes controlling cell growth and division. Through its position in the PP2A complex PPP2R1A moderates phosphorylation status of target proteins impacting diverse cellular activities.
- Pathways
PPP2R1A participates in pathways like the Wnt signaling and the MAPK pathways acting as a brake on kinase-driven signaling. In the Wnt signaling pathway PP2A mediates beta-catenin degradation which influences gene expression related to cell growth. Within the MAPK pathway PPP2R1A interacts with kinases to modulate cellular responses to growth factors. Proteins such as AXIN1 in the Wnt signaling context are associated with the regulatory effects mediated by PPP2R1A.
- Associated diseases and disorders
Alterations in PPP2R1A have been implicated in cancers like ovarian and uterine serous carcinoma. Mutations within this gene can disrupt PP2A activity leading to unchecked cell proliferation. Associations with beta-catenin highlight a significant role in tumorigenesis. In these contexts PPP2R1A's relationship with proteins such as beta-catenin and other components of the Wnt pathway indicates its importance in maintaining cellular homeostasis and its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-PPP2R1A antibody (ab154551)
7.5% SDS PAGE
All lanes: Western blot - Anti-PPP2R1A antibody (ab154551) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 65 kDa
Western blot - Anti-PPP2R1A antibody (ab154551)
7.5% SDS PAGE
All lanes: Western blot - Anti-PPP2R1A antibody (ab154551) at 1/5000 dilution
Lane 1: NIH-3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Lane 3: BCL-1 whole cell lysate at 30 µg
Predicted band size: 65 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody (ab154551)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling PPP2R1A with ab154551 antibody at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R1A antibody (ab154551)
Immunohistochemical analysis of paraffin-embedded U87 xenograft labeling PPP2R1A with ab154551 antibody at 1/500 dilution.
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