AB245622

Anti-PPP2R1A antibody

Name: Anti-PPP2R1A antibody (ab245622) | Abcam
Brand: Abcam Limited
SKU: AB245622

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(1 Publication)

Rabbit Polyclonal PPP2R1A antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 500 to C-terminus.

View Alternative Names

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, PP2Aa, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PPP2R1A

2 Images

Supplier Data

Immunoprecipitation - Anti-PPP2R1A antibody (AB245622)

PPP2R1A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245622 at 3 μg/reaction. Western blot was performed on the immunoprecipitate using ab245622 at 1 μg/ml.

Lane 1 : ab245622 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PPP2R1A antibody (ab245622)

Predicted band size: 65 kDa

false

Supplier Data

Western blot - Anti-PPP2R1A antibody (AB245622)

All lanes:

Western blot - Anti-PPP2R1A antibody (ab245622) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 65 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P30153

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245622 was affinity purified using an epitope specific to PPP2R1A immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 2 scaffold subunit A alpha isoform (PPP2R1A) functions as an important regulatory component of the serine/threonine phosphatase PP2A complex. Alternate names for PPP2R1A include PR65A and Aalpha. This protein has a mass of approximately 65 kDa and is widely expressed in many tissues including the brain heart and lungs. It serves as a scaffold for regulatory and catalytic subunits facilitating their assembly into the active enzyme complex.

Biological function summary

The PP2A complex plays a role in cell cycle control apoptosis and signal transduction. PPP2R1A forms a dimer with one catalytic and one regulatory subunit. This arrangement influences various cell signaling processes controlling cell growth and division. Through its position in the PP2A complex PPP2R1A moderates phosphorylation status of target proteins impacting diverse cellular activities.

Pathways

PPP2R1A participates in pathways like the Wnt signaling and the MAPK pathways acting as a brake on kinase-driven signaling. In the Wnt signaling pathway PP2A mediates beta-catenin degradation which influences gene expression related to cell growth. Within the MAPK pathway PPP2R1A interacts with kinases to modulate cellular responses to growth factors. Proteins such as AXIN1 in the Wnt signaling context are associated with the regulatory effects mediated by PPP2R1A.

Alterations in PPP2R1A have been implicated in cancers like ovarian and uterine serous carcinoma. Mutations within this gene can disrupt PP2A activity leading to unchecked cell proliferation. Associations with beta-catenin highlight a significant role in tumorigenesis. In these contexts PPP2R1A's relationship with proteins such as beta-catenin and other components of the Wnt pathway indicates its importance in maintaining cellular homeostasis and its potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit (PubMed : 15525651, PubMed : 16580887, PubMed : 33243860, PubMed : 33633399, PubMed : 34004147, PubMed : 8694763). Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (PubMed : 15525651). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (PubMed : 16580887). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes (PubMed : 18782753, PubMed : 33633399). STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling (PubMed : 18782753, PubMed : 33633399). Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (PubMed : 18782753, PubMed : 33633399). Key mediator of a quality checkpoint during transcription elongation as part of the Integrator-PP2A (INTAC) complex (PubMed : 33243860, PubMed : 34004147). The INTAC complex drives premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation : within the INTAC complex, acts as a scaffolding subunit for PPP2CA, which catalyzes dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, thereby preventing transcriptional elongation (PubMed : 33243860, PubMed : 34004147). Regulates the recruitment of the SKA complex to kinetochores (PubMed : 28982702).

See full target information Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxicology research 13:tfae096 PubMed38957783

2024

LncRNA HILPDA promotes contrast-induced acute kidney injury by recruiting eIF4B to upregulate XPO1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Wei,Tuersun Tilakezi,Wei Feng,Heyin Yang,Shujun Yang

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Product promise

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Anti-PPP2R1A antibody

Immunoprecipitation - Anti-PPP2R1A antibody (AB245622)

Western blot - Anti-PPP2R1A antibody (AB245622)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise