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AB28350

Anti-PPP2R1A + PPP2R1B antibody

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(2 Publications)

Sheep Polyclonal Ppp2r1a antibody. Suitable for WB and reacts with Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
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Western blot - Anti-PPP2R1A + PPP2R1B antibody (AB28350)
  • WB

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Western blot - Anti-PPP2R1A + PPP2R1B antibody (AB28350)

PPP2R1B detected in total rat brain homogenate with ab28350

All lanes:

Western blot - Anti-PPP2R1A + PPP2R1B antibody (ab28350)

Predicted band size: 65 kDa

Observed band size: 67 kDa

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Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P30153

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 7.4 Preservative: 0.08% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 2 scaffold subunits PPP2R1A and PPP2R1B also known as PR65A alpha and beta isoforms form part of the serine/threonine phosphatase PP2A holoenzyme. These subunits have masses of approximately 65 kDa each. PPP2R1A and PPP2R1B form a structural core that anchors the catalytic and regulatory subunits of PP2A controlling its activity and specificity. These proteins have a broad expression pattern being found in various tissues indicating their essential role in cellular function.
Biological function summary

PPP2R1A + PPP2R1B's function as scaffolding subunits is vital for the assembly of the PP2A enzyme complex. They participate in regulating multiple cellular processes such as cell growth division and apoptosis. The holoenzyme complex including catalytic and regulatory subunits exerts control over the dephosphorylation of a wide range of substrates affecting many cellular functions. These scaffolding proteins help in maintaining cell homeostasis through their regulatory roles.

Pathways

PPP2R1A and PPP2R1B are major components in the control of signal transduction pathways such as the MAPK and Wnt pathways. The MAPK pathway involves interactions with proteins like Raf and MEK while the Wnt pathway impacts beta-catenin stability. By being part of PP2A these proteins are involved in important phosphatase activities influencing cellular responses to various stimuli and maintaining cellular signaling balance.

Mutations or altered expression of PPP2R1A and PPP2R1B are associated with specific cancers such as endometrial and colorectal cancer. Misregulation of these proteins can result in improper cell cycle control and failure of apoptosis providing a growth advantage to cancerous cells. Interactions with proteins such as BCL2 related to apoptosis pathways emphasize their influence on tumorigenesis. By understanding their disease linkage scientists can explore therapeutic targets for cancer treatment.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Ppp2r1a

Additional targets

Ppp2r1b
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 424:1-11 PubMed26780722

2016

Quantitative proteomics reveals novel protein interaction partners of PP2A catalytic subunit in pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangmin Zhang,Divyasri Damacharla,Danjun Ma,Yue Qi,Rebecca Tagett,Sorin Draghici,Anjaneyulu Kowluru,Zhengping Yi

Nature 517:94-98 PubMed25487150

2014

A PP1-PP2A phosphatase relay controls mitotic progression.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Grallert,Elvan Boke,Anja Hagting,Ben Hodgson,Yvonne Connolly,John R Griffiths,Duncan L Smith,Jonathon Pines,Iain M Hagan
View all publications
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