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Rabbit Recombinant Monoclonal PPP2R1B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (AB154815), expandable thumbnail
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815), expandable thumbnail
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815), expandable thumbnail
  • Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Not recommended
Expected
Expected
Rat
Not recommended
Tested
Not recommended
Expected
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.

Alternative names

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PPP2R1B

Recommended products

Rabbit Recombinant Monoclonal PPP2R1B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10158
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein PPP2R1B also known as Protein Phosphatase 2 Scaffold Subunit A beta isoform acts as a scaffold for the assembly of PP2A complexes. PPP2R1B has a molecular mass of approximately 66 kDa. The protein expresses widely across various tissues with notable presence in the brain heart and skeletal muscle. As a scaffold subunit PPP2R1B coordinates the structural integrity necessary for phosphatase activity by recruiting regulatory and catalytic subunits important to its function.

Biological function summary

PPP2R1B integrates into the PP2A holoenzyme complex a major serine/threonine phosphatase. This complex regulates numerous cellular processes including cell growth division and signal transduction. PPP2R1B plays a role in maintaining cellular homeostasis through its regulatory activities. By doing so it links to various signaling events some of which control oncogenes and tumor suppressors demonstrating the protein's influence over cell cycle regulation and apoptosis.

Pathways

PPP2R1B plays a significant role in the Raf/MEK/ERK signaling pathway and the PI3K/AKT pathway. Through these pathways PPP2R1B interacts with PDPK1 and AKT1 modulating their phosphorylation status and thereby affecting cell proliferation and survival. By influencing such pathways PPP2R1B helps determine cellular responses to external stimuli impacting processes like differentiation and metabolism that are vital for organismal function.

Associated diseases and disorders

PPP2R1B links to cancer and Alzheimer's disease. Mutations or downregulation in PPP2R1B have been implicated in colorectal cancer where it alters the PP2A activity and allows unregulated cellular proliferation. In Alzheimer's disease PPP2R1B's interaction with tau protein goes awry contributing to neurofibrillary tangle formation. These connections highlight the importance of PPP2R1B in understanding the molecular basis of these diseases and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815), expandable thumbnail

    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Lanes 1-4: Merged signal (red and green). Green - ab154815 observed at 66 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab154815 Anti-PPP2R1B antibody [EPR10158] was shown to specifically react with PPP2R1B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human PPP2R1B knockout HEK-293T cell line ab266729 (knockout cell lysate Human PPP2R1B knockout HEK-293T cell lysate ab258133) was used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: PPP2R1B knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human PPP2R1B knockout HEK-293T cell line (Human PPP2R1B knockout HEK-293T cell line ab266729)

    Lane 3: Caco-2 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 66 kDa

    Observed band size: 66 kDa

  • Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (ab154815), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Intracellular Flow Cytometry analysis of Caco-2 (human colorectal adenocarcinoma) cells labeling PPP2R1B with unpurified ab154815 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815), expandable thumbnail

    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: PPP2R1B knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: Caco2 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab154815 observed at 67 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab154815 was shown to specifically react with PPP2R1B when PPP2R1B knockout samples were used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Predicted band size: 66 kDa

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815), expandable thumbnail

    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    All lanes: Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG) at 1/2000 dilution

    Predicted band size: 66 kDa

    Observed band size: 66 kDa

    Exposure time: 3min

  • Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815), expandable thumbnail

    Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    All lanes: Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 10 µg

    Lane 2: Caco 2 cell lysate at 10 µg

    Predicted band size: 66 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (ab154815), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (ab154815)

    Immunofluorescence analysis of Caco 2 cells, labeling PPP2R1B using ab154815 at 1/50 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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