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AB154815

Anti-PPP2R1B antibody [EPR10158]

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(3 Publications)

Rabbit Recombinant Monoclonal PPP2R1B antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform, PP2A subunit A isoform PR65-beta, PP2A subunit A isoform R1-beta, PPP2R1B

6 Images
Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (AB154815)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-PPP2R1B antibody [EPR10158] (AB154815)

Intracellular Flow Cytometry analysis of Caco-2 (human colorectal adenocarcinoma) cells labeling PPP2R1B with unpurified ab154815 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (AB154815)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1B antibody [EPR10158] (AB154815)

Immunofluorescence analysis of Caco 2 cells, labeling PPP2R1B using ab154815 at 1/50 dilution.

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)
  • WB

Lab

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : PPP2R1B knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : Caco2 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab154815 observed at 67 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab154815 was shown to specifically react with PPP2R1B when PPP2R1B knockout samples were used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815)

Predicted band size: 66 kDa

false

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)
  • WB

Unknown

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)

All lanes:

Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Caco 2 cell lysate at 10 µg

Predicted band size: 66 kDa

false

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)
  • WB

Lab

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)

Lanes 1-4 : Merged signal (red and green). Green - ab154815 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.

ab154815 Anti-PPP2R1B antibody [EPR10158] was shown to specifically react with PPP2R1B in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266729 (knockout cell lysate ab258133) was used. Wild-type and PPP2R1B knockout samples were subjected to SDS-PAGE. ab154815 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

PPP2R1B knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human PPP2R1B knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ppp2r1b-knockout-hek-293t-cell-line-ab266729'>ab266729</a>)

Lane 3:

Caco-2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)
  • WB

Lab

Western blot - Anti-PPP2R1B antibody [EPR10158] (AB154815)

All lanes:

Western blot - Anti-PPP2R1B antibody [EPR10158] (ab154815) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG) at 1/2000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Exposure time: 3min

  • 578 PE

    PE Anti-PPP2R1B antibody [EPR10158]

  • 660 APC

    APC Anti-PPP2R1B antibody [EPR10158]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-PPP2R1B antibody [EPR10158]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-PPP2R1B antibody [EPR10158]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-PPP2R1B antibody [EPR10158]

  • Carrier free

    Anti-PPP2R1B antibody [EPR10158] - BSA and Azide free

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-PPP2R1B antibody [EPR10158]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-PPP2R1B antibody [EPR10158]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10158

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PPP2R1B also known as Protein Phosphatase 2 Scaffold Subunit A beta isoform acts as a scaffold for the assembly of PP2A complexes. PPP2R1B has a molecular mass of approximately 66 kDa. The protein expresses widely across various tissues with notable presence in the brain heart and skeletal muscle. As a scaffold subunit PPP2R1B coordinates the structural integrity necessary for phosphatase activity by recruiting regulatory and catalytic subunits important to its function.
Biological function summary

PPP2R1B integrates into the PP2A holoenzyme complex a major serine/threonine phosphatase. This complex regulates numerous cellular processes including cell growth division and signal transduction. PPP2R1B plays a role in maintaining cellular homeostasis through its regulatory activities. By doing so it links to various signaling events some of which control oncogenes and tumor suppressors demonstrating the protein's influence over cell cycle regulation and apoptosis.

Pathways

PPP2R1B plays a significant role in the Raf/MEK/ERK signaling pathway and the PI3K/AKT pathway. Through these pathways PPP2R1B interacts with PDPK1 and AKT1 modulating their phosphorylation status and thereby affecting cell proliferation and survival. By influencing such pathways PPP2R1B helps determine cellular responses to external stimuli impacting processes like differentiation and metabolism that are vital for organismal function.

PPP2R1B links to cancer and Alzheimer's disease. Mutations or downregulation in PPP2R1B have been implicated in colorectal cancer where it alters the PP2A activity and allows unregulated cellular proliferation. In Alzheimer's disease PPP2R1B's interaction with tau protein goes awry contributing to neurofibrillary tangle formation. These connections highlight the importance of PPP2R1B in understanding the molecular basis of these diseases and potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
See full target information Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 12:720619 PubMed34512348

2021

The Downregulation of eIF3a Contributes to Vemurafenib Resistance in Melanoma by Activating ERK via PPP2R1B.

Applications

Unspecified application

Species

Unspecified reactive species

Shi-Long Jiang,Zhi-Bin Wang,Tao Zhu,Ting Jiang,Jiang-Feng Fei,Chong Liu,Chao Luo,Yan Cheng,Zhao-Qian Liu

Journal of cell science 133: PubMed32591484

2020

PP2A--B55γ counteracts Cdk1 and regulates proper spindle orientation through the cortical dynein adaptor NuMA.

Applications

Unspecified application

Species

Unspecified reactive species

Riya Keshri,Ashwathi Rajeevan,Sachin Kotak

Cellular and molecular gastroenterology and hepatology 2:281-301.e9 PubMed28174720

2016

Netrin-1 Protects Hepatocytes Against Cell Death Through Sustained Translation During the Unfolded Protein Response.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Lahlali,Marie-Laure Plissonnier,Cristina Romero-López,Maud Michelet,Benjamin Ducarouge,Alfredo Berzal-Herranz,Fabien Zoulim,Patrick Mehlen,Romain Parent
View all publications
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