Anti-PPP2R2A antibody [RP23040118]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R2A Antibody [RP23040118] (AB313413)

Immunofluorescent analysis of HeLa cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permeabilized with 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous PPP2R2A (PP2AB) with ab313413 at 5 µg/mL concentration followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of PPP2R2A protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) is a composite image of Panels a and b clearly demonstrating centromere localization of PP2AB. The images were captured at 60X magnification.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R2A Antibody [RP23040118] (AB313413)

Immunofluorescent analysis of HeLa cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permeabilized with 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous PPP2R2A (PP2AB) with ab313413 at 5 µg/mL concentration followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of PPP2R2A protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of PPP2R2A. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

• WB

Supplier Data

Western blot - Anti-PPP2R2A Antibody [RP23040118] (AB313413)

All lanes:

Western blot - Anti-PPP2R2A Antibody [RP23040118] (ab313413) at 2.5 µg/mL

Lane 1:

C2C12 membrane enriched extract at 30 µg

Lane 2:

NIH/3T3 membrane enriched extract at 30 µg

Lane 3:

Mouse testis tissue lysate at 30 µg

Lane 4:

Mouse brain tissue lysate at 30 µg

Lane 5:

Rat brain tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Observed band size: 36 kDa

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