Rabbit Polyclonal PPP2R2B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP2R2B aa 50-150.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PPP2R2B aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q00005
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/199.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Rat | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Cow, Pig, Xenopus laevis, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance (By similarity).
Alternative names
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, PP2A subunit B isoform B55-beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A subunit B isoform beta, PPP2R2B
Recommended products
Rabbit Polyclonal PPP2R2B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP2R2B aa 50-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PPP2R2B aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q00005
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein phosphatase 2 regulatory subunit B also known as PPP2R2B or B beta regulatory subunit plays an important role in cellular processes. It has a molecular mass of approximately 55 kDa. This subunit is part of the protein phosphatase 2 (PP2A) complex and is expressed widely but found at high levels in the brain. In particular its expression is prominent in specific regions such as the cerebellum.
- Biological function summary
PPP2R2B acts as a regulatory component of the PP2A holoenzyme complex. PP2A is a serine/threonine phosphatase involved in the negative control of cell growth and division. PPP2R2B is one of the regulatory subunits that directs the PP2A core enzyme to certain substrates influencing dephosphorylation activities. It participates in maintaining normal cellular functions especially within the nervous system by modulating the phosphorylation state of various proteins.
- Pathways
PPP2R2B impacts the cell cycle and PI3K/AKT signaling pathways. It negatively regulates these pathways by dephosphorylating target proteins acting as a tumor suppressor. The regulation by PPP2R2B of the PI3K/AKT pathway links it to controlling cell proliferation and survival interacting with proteins like AKT1 and mTOR. The involvement of PPP2R2B in these pathways reflects its pivotal role in ensuring cellular homeostasis and response to extracellular signals.
- Associated diseases and disorders
PPP2R2B is associated with neurodegenerative conditions including spinocerebellar ataxia type 12 and Huntington’s disease. Abnormalities in PPP2R2B function or expression can disrupt normal neuronal activities leading to these disorders. In Huntington’s disease alterations in PPP2R2B contribute to the pathology by affecting the dephosphorylation dynamics of proteins such as huntingtin exacerbating disease symptoms and progression. Understanding the modulation of PPP2R2B in diseases can offer insights into potential therapeutic targets for these debilitating conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R2B antibody (ab251885)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PPP2R2B (green) using ab251885 at 4 µg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R2B antibody (ab251885)
Paraffin-embedded human cerebral cortex tissue stained for PPP2R2B using ab251885 at 1/200 dilution in immunohistochemical analysis.
Western blot - Anti-PPP2R2B antibody (ab251885)
All lanes: Western blot - Anti-PPP2R2B antibody (ab251885) at 0.4 µg/mL
Lane 1: RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2: U-251 MG (human brain glioma cell line) whole cell lysate
Lane 3: Human plasma
Lane 4: Human liver lysate
Lane 5: Human tonsil lysate
Predicted band size: 52 kDa
Western blot - Anti-PPP2R2B antibody (ab251885)
All lanes: Western blot - Anti-PPP2R2B antibody (ab251885) at 0.4 µg/mL
Lane 1: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2: NBT-II (rat Wistar bladder tumor cell line) whole cell lysate
Predicted band size: 52 kDa
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