AB157461

Anti-PPP2R2B antibody [EPR10961]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PPP2R2B antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 2 publications.

View Alternative Names

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, PP2A subunit B isoform B55-beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A subunit B isoform beta, PPP2R2B

4 Images

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Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

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Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/2000 dilution

Lane 1:

Mouse kidney lysate at 20 µg

Lane 2:

Mouse spleen lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

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Lab

Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM /TBST

Exposure time : 3.25 seconds

Recombinant Human PPP2R2B protein (Cat# : ab132644)

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/1000 dilution

All lanes:

GST-tagged Recombinant Human PPP2R2B protein (aa 1 to 443) 15ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 76 kDa

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Unknown

Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (ab157461) at 1/2000 dilution

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10961

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody may also recognize PPP2R2A and PPP2R2D based on our internal QC data.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 2 regulatory subunit B also known as PPP2R2B or B beta regulatory subunit plays an important role in cellular processes. It has a molecular mass of approximately 55 kDa. This subunit is part of the protein phosphatase 2 (PP2A) complex and is expressed widely but found at high levels in the brain. In particular its expression is prominent in specific regions such as the cerebellum.

Biological function summary

PPP2R2B acts as a regulatory component of the PP2A holoenzyme complex. PP2A is a serine/threonine phosphatase involved in the negative control of cell growth and division. PPP2R2B is one of the regulatory subunits that directs the PP2A core enzyme to certain substrates influencing dephosphorylation activities. It participates in maintaining normal cellular functions especially within the nervous system by modulating the phosphorylation state of various proteins.

Pathways

PPP2R2B impacts the cell cycle and PI3K/AKT signaling pathways. It negatively regulates these pathways by dephosphorylating target proteins acting as a tumor suppressor. The regulation by PPP2R2B of the PI3K/AKT pathway links it to controlling cell proliferation and survival interacting with proteins like AKT1 and mTOR. The involvement of PPP2R2B in these pathways reflects its pivotal role in ensuring cellular homeostasis and response to extracellular signals.

PPP2R2B is associated with neurodegenerative conditions including spinocerebellar ataxia type 12 and Huntington’s disease. Abnormalities in PPP2R2B function or expression can disrupt normal neuronal activities leading to these disorders. In Huntington’s disease alterations in PPP2R2B contribute to the pathology by affecting the dephosphorylation dynamics of proteins such as huntingtin exacerbating disease symptoms and progression. Understanding the modulation of PPP2R2B in diseases can offer insights into potential therapeutic targets for these debilitating conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance (By similarity).

See full target information PPP2R2B

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:292 PubMed38976080

2024

Nuclear translocation of ISG15 regulated by PPP2R2B inhibits cisplatin resistance of bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gaowei Huang,Jinwen Liu,Anze Yu,Chenggong Luo,Jiangquan Zhu,Yinghan Wang,Ziran Dai,Lizhen Zhang,Zihao Feng,Jun Lu,Zhong Dong,Junhang Luo,Wei Chen,Zhenhua Chen

Cells 11: PubMed36139424

2022

Lack of ZNF365 Drives Senescence and Exacerbates Experimental Lung Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Urista,Mariel Maldonado,Fernanda Toscano-Marquez,Remedios Ramírez,Yalbi Itzel Balderas-Martínez,Carina Becerril,Yair Romero,Moisés Selman,Annie Pardo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP2R2B antibody [EPR10961]

Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

Western blot - Anti-PPP2R2B antibody [EPR10961] (AB157461)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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