AB249330

Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal PPP2R2B antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Recombinant fragment - Human samples.

View Alternative Names

Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, PP2A subunit B isoform B55-beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A subunit B isoform beta, PPP2R2B

2 Images

Lab

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

This data was developed using ab157461, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3.25 seconds.

Recombinant Human PPP2R2B protein (Cat

: ab132644).

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (<a href='/en-us/products/primary-antibodies/ppp2r2b-antibody-epr10961-ab157461'>ab157461</a>) at 1/1000 dilution

All lanes:

GST-tagged Recombinant Human PPP2R2B protein (aa 1 to 443) 15ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 76 kDa

false

Unknown

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

This data was developed using ab157461, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPP2R2B antibody [EPR10961] (<a href='/en-us/products/primary-antibodies/ppp2r2b-antibody-epr10961-ab157461'>ab157461</a>) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Lane 5:

Human fetal brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 52 kDa

false

Unconjugated

Anti-PPP2R2B antibody [EPR10961]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10961

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The antibody may also recognize PPP2R2A and PPP2R2D based on our internal QC data.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab249330 is the carrier-free version of ab157461.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 2 regulatory subunit B also known as PPP2R2B or B beta regulatory subunit plays an important role in cellular processes. It has a molecular mass of approximately 55 kDa. This subunit is part of the protein phosphatase 2 (PP2A) complex and is expressed widely but found at high levels in the brain. In particular its expression is prominent in specific regions such as the cerebellum.

Biological function summary

PPP2R2B acts as a regulatory component of the PP2A holoenzyme complex. PP2A is a serine/threonine phosphatase involved in the negative control of cell growth and division. PPP2R2B is one of the regulatory subunits that directs the PP2A core enzyme to certain substrates influencing dephosphorylation activities. It participates in maintaining normal cellular functions especially within the nervous system by modulating the phosphorylation state of various proteins.

Pathways

PPP2R2B impacts the cell cycle and PI3K/AKT signaling pathways. It negatively regulates these pathways by dephosphorylating target proteins acting as a tumor suppressor. The regulation by PPP2R2B of the PI3K/AKT pathway links it to controlling cell proliferation and survival interacting with proteins like AKT1 and mTOR. The involvement of PPP2R2B in these pathways reflects its pivotal role in ensuring cellular homeostasis and response to extracellular signals.

PPP2R2B is associated with neurodegenerative conditions including spinocerebellar ataxia type 12 and Huntington’s disease. Abnormalities in PPP2R2B function or expression can disrupt normal neuronal activities leading to these disorders. In Huntington’s disease alterations in PPP2R2B contribute to the pathology by affecting the dephosphorylation dynamics of proteins such as huntingtin exacerbating disease symptoms and progression. Understanding the modulation of PPP2R2B in diseases can offer insights into potential therapeutic targets for these debilitating conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity (By similarity). Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance (By similarity).

See full target information PPP2R2B

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

Western blot - Anti-PPP2R2B antibody [EPR10961] - BSA and Azide free (AB249330)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise