AB181071

Anti-PPP2R2D antibody [EPR13624]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PPP2R2D antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.

View Alternative Names

KIAA1541, PPP2R2D, Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform, PP2A subunit B isoform B55-delta, PP2A subunit B isoform PR55-delta, PP2A subunit B isoform R2-delta, PP2A subunit B isoform delta

4 Images

Unknown

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Unknown

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Unknown

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

Blocking Buffer : 5% NFDM/TBST

Dilution Buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/5000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Supplier Data

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (ab181071) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Lane 3:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13624

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PPP2R2D protein also known as B regulatory subunit of phosphatase 2A (B55-Delta) is a part of the protein phosphatase 2 (PP2A) complex. It is an important element of the holoenzyme PP2A with an approximate molecular mass of 55 kilodaltons. This protein is expressed widely in human tissues including the brain and liver. PPP2R2D functions by regulating PP2A activity affecting various substrates and controlling the dephosphorylation process.

Biological function summary

PPP2R2D plays a role in cell cycle regulation and apoptosis. As a regulatory subunit of the PP2A complex it modulates the enzyme’s activity and substrate specificity. This control makes PPP2R2D important in the mitotic spindle checkpoint facilitating proper chromosome segregation. Its influence on cell cycle arrest and apoptosis emerges from its ability to target several key signaling molecules thereby ensuring proper cell function and survival.

Pathways

The PP2A complex containing PPP2R2D is integral to the MAPK/ERK and PI3K/AKT pathways. Through these pathways PPP2R2D influences cellular processes like growth differentiation and survival. Related proteins in these pathways include RAF and AKT which interact with PP2A peptides to stabilize the signaling environment. PPP2R2D's functionality ensures the pathways remain balanced enabling cells to maintain normal physiological functions.

Aberrant expression or mutation of PPP2R2D associates with cancers and neurodegenerative diseases. In cancer altered PP2A activity often linked with related proteins such as MYC contributes to uncontrolled cell proliferation. Similarly in neurological conditions like Alzheimer's disease the dysregulation of PP2A-related processes can lead to tau hyperphosphorylation highlighting deficiencies in the apoptotic pathways. Understanding PPP2R2D's role can therefore highlight potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle : the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and may also direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).

See full target information PPP2R2D

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 5: PubMed32897879

2020

PPP2R2D suppresses IL-2 production and Treg function.

Applications

Unspecified application

Species

Unspecified reactive species

Wenliang Pan,Amir Sharabi,Andrew Ferretti,Yinfeng Zhang,Catalina Burbano,Nobuya Yoshida,Maria G Tsokos,George C Tsokos

International journal of oncology 52:2011-2020 PubMed29568966

2018

PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation.

Applications

Unspecified application

Species

Unspecified reactive species

Shijun Yu,Li Li,Qiong Wu,Ning Dou,Yandong Li,Yong Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP2R2D antibody [EPR13624]

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

Western blot - Anti-PPP2R2D antibody [EPR13624] (AB181071)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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