JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250325

Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PPP2R2D antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

KIAA1541, PPP2R2D, Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform, PP2A subunit B isoform B55-delta, PP2A subunit B isoform PR55-delta, PP2A subunit B isoform R2-delta, PP2A subunit B isoform delta

4 Images
Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)
  • WB

Unknown

Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)

This data was developed using ab181071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (<a href='/en-us/products/primary-antibodies/ppp2r2d-antibody-epr13624-ab181071'>ab181071</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 20 µg

Lane 2:

Human fetal brain tissue lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)
  • WB

Unknown

Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)

This data was developed using ab181071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (<a href='/en-us/products/primary-antibodies/ppp2r2d-antibody-epr13624-ab181071'>ab181071</a>) at 1/5000 dilution

All lanes:

Mouse brain tissue lysate

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)
  • WB

Unknown

Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)

This data was developed using ab181071, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (<a href='/en-us/products/primary-antibodies/ppp2r2d-antibody-epr13624-ab181071'>ab181071</a>) at 1/5000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)
  • WB

Supplier Data

Western blot - Anti-PPP2R2D antibody [EPR13624] - BSA and Azide free (AB250325)

This data was developed using ab181071, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPP2R2D antibody [EPR13624] (<a href='/en-us/products/primary-antibodies/ppp2r2d-antibody-epr13624-ab181071'>ab181071</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

SH-SY5Y cell lysate at 20 µg

Lane 3:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13624

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB181152

Anti-ZCCHC8 antibody [EPR13612]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-ZCCHC8 antibody [EPR13612] (AB181152)

  • 0
  • 0
Primary Antibodies

AB174294

Anti-WDTC1 antibody [EPR12504]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WDTC1 antibody [EPR12504] (AB174294)

  • 0
  • 0
Primary Antibodies

AB134047

Anti-VCAM1 antibody [EPR5047]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-VCAM1 antibody [EPR5047] (AB134047)

  • 5
  • 17
Primary Antibodies

AB109235

Anti-Xanthine Oxidase antibody [EPR4605]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (AB109235)

  • 4
  • 3
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

Product details

ab250325 is the carrier-free version of ab181071.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PPP2R2D protein also known as B regulatory subunit of phosphatase 2A (B55-Delta) is a part of the protein phosphatase 2 (PP2A) complex. It is an important element of the holoenzyme PP2A with an approximate molecular mass of 55 kilodaltons. This protein is expressed widely in human tissues including the brain and liver. PPP2R2D functions by regulating PP2A activity affecting various substrates and controlling the dephosphorylation process.
Biological function summary

PPP2R2D plays a role in cell cycle regulation and apoptosis. As a regulatory subunit of the PP2A complex it modulates the enzyme’s activity and substrate specificity. This control makes PPP2R2D important in the mitotic spindle checkpoint facilitating proper chromosome segregation. Its influence on cell cycle arrest and apoptosis emerges from its ability to target several key signaling molecules thereby ensuring proper cell function and survival.

Pathways

The PP2A complex containing PPP2R2D is integral to the MAPK/ERK and PI3K/AKT pathways. Through these pathways PPP2R2D influences cellular processes like growth differentiation and survival. Related proteins in these pathways include RAF and AKT which interact with PP2A peptides to stabilize the signaling environment. PPP2R2D's functionality ensures the pathways remain balanced enabling cells to maintain normal physiological functions.

Aberrant expression or mutation of PPP2R2D associates with cancers and neurodegenerative diseases. In cancer altered PP2A activity often linked with related proteins such as MYC contributes to uncontrolled cell proliferation. Similarly in neurological conditions like Alzheimer's disease the dysregulation of PP2A-related processes can lead to tau hyperphosphorylation highlighting deficiencies in the apoptotic pathways. Understanding PPP2R2D's role can therefore highlight potential therapeutic targets for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Substrate-recognition subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. Involved in chromosome clustering during late mitosis by mediating dephosphorylation of MKI67 (By similarity). The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle : the activity is high in interphase and low in mitosis (By similarity).
See full target information PPP2R2D
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com