AB72027

Anti-PPP2R3B antibody

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal PPP2R3B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP2R3B.

View Alternative Names

PPP2R3L, PPP2R3B, Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta, PP2A subunit B isoform PR48, Protein phosphatase 2A 48 kDa regulatory subunit

2 Images

Unknown

Immunoprecipitation - Anti-PPP2R3B antibody (AB72027)

Immunoprecipitation/ Western Blot of PPP2R3B.
Lane 1 : ab72027 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
For western blot detection step ab72027 was used at 1 μg/ml.
Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PPP2R3B antibody (ab72027)

Predicted band size: 65 kDa

false

Unknown

Western blot - Anti-PPP2R3B antibody (AB72027)

All lanes:

Western blot - Anti-PPP2R3B antibody (ab72027) at 0.1 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Lane 3:

Whole cell lysate from Hela cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 65 kDa

Observed band size: 200 kDa,65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PPP2R3B. The exact immunogen used to generate this antibody is proprietary information.

Q9Y5P8

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP2R3B also known as Protein Phosphatase 2 Regulatory Subunit B'' is a regulatory subunit of the protein phosphatase 2A (PP2A) complex. This protein plays a role in modulating the activity of the PP2A enzyme complex which is a critical enzyme in cell signaling. PPP2R3B has a molecular weight of approximately 57 kDa. It is expressed in various tissues with notable expression in the heart and skeletal muscle suggesting its importance in these tissues. The regulatory function of PPP2R3B involves interaction with the catalytic and scaffold subunits of PP2A.

Biological function summary

PPP2R3B influences a variety of cellular processes through its role in the PP2A complex. PP2A is involved in cell growth division and apoptosis by regulating phosphorylation states of serine and threonine residues on target proteins. As a regulatory subunit PPP2R3B can modify the specificity and localization of the PP2A complex impacting cellular signaling pathways. It does not act alone but as part of a larger protein complex it helps ensure accurate and timely signal transduction within cells.

Pathways

The activity of PPP2R3B within the PP2A complex influences the Wnt and MAPK signaling pathways. These pathways are important in regulating cell fate decisions and proliferative responses. Within the Wnt pathway PPP2R3B associates with proteins like Axin and GSK-3β influencing the stability and activity of β-catenin. In the MAPK pathway it can impact the activation state of key signaling proteins such as Raf-1 linking it with cell proliferation and differentiation.

Dysregulation of PPP2R3B has implications in cancer and cardiac disorders. In certain cancers altered expression or mutations can disrupt normal cell proliferation control contributing to tumorigenesis. The interaction of PPP2R3B with proteins such as MYC is evident in some cancer contexts as it affects MYC stabilization and function. In cardiac disorders changes in PP2A complex activity influenced by PPP2R3B can lead to improper cardiac development or function connecting it to diseases like cardiomyopathies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

See full target information PPP2R3B

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 140:645-655.e6 PubMed31472105

2019

Innate Immune Dysfunction in Rosacea Promotes Photosensitivity and Vascular Adhesion Molecule Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Nikhil N Kulkarni,Toshiya Takahashi,James A Sanford,Yun Tong,Adrian F Gombart,Brian Hinds,Joyce Y Cheng,Richard L Gallo

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP2R3B antibody

Immunoprecipitation - Anti-PPP2R3B antibody (AB72027)

Western blot - Anti-PPP2R3B antibody (AB72027)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise