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AB247135

Anti-PPP2R5A antibody

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Rabbit Polyclonal PPP2R5A antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP2R5A aa 100-150.

View Alternative Names

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform PR61-alpha, PP2A B subunit isoform R5-alpha, PR61alpha, PPP2R5A

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5A antibody (AB247135)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5A antibody (AB247135)

PFA fixed, Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling PPP2R5A using ab247135 at 4 μg/ml (green) in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PPP2R5A aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q15172

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP2R5A also known as B56α is a regulatory subunit of protein phosphatase 2A (PP2A) with a molecular weight of approximately 55 kDa. This protein subunit expresses widely in various tissues indicating its important role in multiple biological processes. B56α mainly localizes in the cytoplasm where it associates with other components of the PP2A holoenzyme to perform its regulatory functions.
Biological function summary

B56α plays an important role in modulating PP2A activity by influencing substrate specificity and subcellular localization. It is part of the heterotrimeric PP2A complex which also includes a catalytic and a scaffolding subunit. This complex serves critical functions in cell cycle regulation development and apoptosis. By binding specific substrates B56α mediates processes like dephosphorylation affecting signal transduction pathways.

Pathways

B56α is involved in the regulation of signaling pathways such as the Akt/mTOR and Wnt signaling pathways. In these pathways it interacts with proteins like Akt and GSK3β modulating their phosphorylation states and activities. By acting within these pathways B56α impacts processes such as cell growth survival and differentiation showcasing its significance in cellular homeostasis.

B56α's dysregulation links to cancer and neurodegenerative diseases. In certain cancers altered PP2A activity can affect tumor suppressor pathways involving proteins like p53 leading to unchecked cell proliferation. In neurodegenerative diseases changes in B56α may affect tau protein phosphorylation state contributing to conditions like Alzheimer's disease. Understanding B56α's function and interactions helps in exploring therapeutic options for these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.
See full target information PPP2R5A
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Product promise

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