AB89621

Anti-PPP2R5A antibody

Name: Anti-PPP2R5A antibody (ab89621) | Abcam
Brand: Abcam Limited
SKU: AB89621
Price: 535 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(9 Publications)

Mouse Polyclonal PPP2R5A antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PPP2R5A.

View Alternative Names

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform, PP2A B subunit isoform B'-alpha, PP2A B subunit isoform B56-alpha, PP2A B subunit isoform PR61-alpha, PP2A B subunit isoform R5-alpha, PR61alpha, PPP2R5A

3 Images

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Western blot - Anti-PPP2R5A antibody (AB89621)

All lanes:

Western blot - Anti-PPP2R5A antibody (ab89621) at 1 µg/mL

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Human placenta tissue lysate at 50 µg

Predicted band size: 56 kDa

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Western blot - Anti-PPP2R5A antibody (AB89621)

All lanes:

Western blot - Anti-PPP2R5A antibody (ab89621) at 1 µg/mL

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 56 kDa

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Unknown

Western blot - Anti-PPP2R5A antibody (AB89621)

All lanes:

Western blot - Anti-PPP2R5A antibody (ab89621) at 1 µg/mL

Lane 1:

PPP2R5A transfected 293T cell lysate at 25 µg

Lane 2:

Untransfected 293T cell lysate at 25 µg

Predicted band size: 56 kDa

Observed band size: 53 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PPP2R5A.

Q15172

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP2R5A also known as B56α is a regulatory subunit of protein phosphatase 2A (PP2A) with a molecular weight of approximately 55 kDa. This protein subunit expresses widely in various tissues indicating its important role in multiple biological processes. B56α mainly localizes in the cytoplasm where it associates with other components of the PP2A holoenzyme to perform its regulatory functions.

Biological function summary

B56α plays an important role in modulating PP2A activity by influencing substrate specificity and subcellular localization. It is part of the heterotrimeric PP2A complex which also includes a catalytic and a scaffolding subunit. This complex serves critical functions in cell cycle regulation development and apoptosis. By binding specific substrates B56α mediates processes like dephosphorylation affecting signal transduction pathways.

Pathways

B56α is involved in the regulation of signaling pathways such as the Akt/mTOR and Wnt signaling pathways. In these pathways it interacts with proteins like Akt and GSK3β modulating their phosphorylation states and activities. By acting within these pathways B56α impacts processes such as cell growth survival and differentiation showcasing its significance in cellular homeostasis.

B56α's dysregulation links to cancer and neurodegenerative diseases. In certain cancers altered PP2A activity can affect tumor suppressor pathways involving proteins like p53 leading to unchecked cell proliferation. In neurodegenerative diseases changes in B56α may affect tau protein phosphorylation state contributing to conditions like Alzheimer's disease. Understanding B56α's function and interactions helps in exploring therapeutic options for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.

See full target information PPP2R5A

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 14:7450-7469 PubMed39659585

2024

Low expression promotes sensitivity to CHK1 inhibition in high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaojun Qiu,Deepika Sigh,Yujie Liu,Chandra B Prasad,Nichalos Bean,Chunhong Yan,Zaibo Li,Xiaoli Zhang,Goutham Narla,Analisa DiFeo,Qi-En Wang,Junran Zhang

Cell biochemistry and biophysics 81:59-68 PubMed36324030

2022

Protein Phosphatase 2a Inhibits Gastric Cancer Cell Glycolysis by Reducing MYC Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenhua Cai,Wei Zhang,Ruiqing Zhou,Yuhong Wang,Yunzhang Feng

Cancer science 112:4593-4603 PubMed34449943

2021

DOC-2/DAB2 interactive protein destabilizes c-Myc to impair the growth and self-renewal of colon tumor-repopulating cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haiou Li,Yunjiao Zhou,Meng Wang,Haizhou Wang,Yangyang Zhang,Ruyi Peng,Ruike Zhang,Meng Zhang,Mengna Zhang,Peishan Qiu,Lan Liu,Qiu Zhao,Jing Liu

Genome medicine 13:116 PubMed34271981

2021

Secondary resistance to anti-EGFR therapy by transcriptional reprogramming in patient-derived colorectal cancer models.

Applications

Unspecified application

Species

Unspecified reactive species

Deepak Vangala,Swetlana Ladigan,Sven T Liffers,Soha Noseir,Abdelouahid Maghnouj,Tina-Maria Götze,Berlinda Verdoodt,Susanne Klein-Scory,Laura Godfrey,Martina K Zowada,Mario Huerta,Daniel L Edelstein,Jaime Martinez de Villarreal,Miriam Marqués,Jörg Kumbrink,Andreas Jung,Tobias Schiergens,Jens Werner,Volker Heinemann,Sebastian Stintzing,Doris Lindoerfer,Ulrich Mansmann,Michael Pohl,Christian Teschendorf,Christiane Bernhardt,Heiner Wolters,Josef Stern,Selami Usta,Richard Viebahn,Jacob Admard,Nicolas Casadei,Stefan Fröhling,Claudia R Ball,Jens T Siveke,Hanno Glimm,Andrea Tannapfel,Wolff Schmiegel,Stephan A Hahn

Cellular and molecular gastroenterology and hepatology 12:1669-1682 PubMed34245919

2021

HDAC1-Dependent Repression of Markers of Hepatocytes and P21 Is Involved in Development of Pediatric Liver Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Rivas,Michael E Johnston,Ruhi Gulati,Meenasri Kumbaji,Talita Ferreira Margues Aguiar,Lubov Timchenko,Ana Krepischi,Soona Shin,Alexander Bondoc,Gregory Tiao,James Geller,Nikolai Timchenko

Molecular oncology 15:3076-3090 PubMed33932092

2021

SF3B1 mutation in pancreatic cancer contributes to aerobic glycolysis and tumor growth through a PP2A-c-Myc axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Yu Yang,Yan-Miao Huo,Min-Wei Yang,Yang Shen,De-Jun Liu,Xue-Liang Fu,Ling-Ye Tao,Rui-Zhe He,Jun-Feng Zhang,Rong Hua,Shu-Heng Jiang,Yong-Wei Sun,Wei Liu

Cancer research 80:3305-3318 PubMed32522823

2020

A Genome-Wide Pooled shRNA Screen Identifies PPP2R2A as a Predictive Biomarker for the Response to ATR and CHK1 Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaojun Qiu,Pengyan Fa,Tao Liu,Chandra B Prasad,Shanhuai Ma,Zhipeng Hong,Ernest R Chan,Hongbing Wang,Zaibo Li,Kai He,Qi-En Wang,Terence M Williams,Chunhong Yan,Steven T Sizemore,Goutham Narla,Junran Zhang

eLife 5: PubMed27690223

2016

Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants.

Applications

Unspecified application

Species

Unspecified reactive species

Edward Jd Greenwood,Nicholas J Matheson,Kim Wals,Dick Jh van den Boomen,Robin Antrobus,James C Williamson,Paul J Lehner

Nature communications 4:2677 PubMed24157919

2013

Bod1 regulates protein phosphatase 2A at mitotic kinetochores.

Applications

Species

Human

Iain M Porter,Katharina Schleicher,Michael Porter,Jason R Swedlow

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PPP2R5A antibody

Western blot - Anti-PPP2R5A antibody (AB89621)

Western blot - Anti-PPP2R5A antibody (AB89621)

Western blot - Anti-PPP2R5A antibody (AB89621)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise