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AB1366

Anti-PPP2R5B antibody

5

(1 Review)

|

(1 Publication)

Goat Polyclonal PPP2R5B antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform aa 450 to C-terminus.

View Alternative Names

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, PP2A B subunit isoform B'-beta, PP2A B subunit isoform B56-beta, PP2A B subunit isoform PR61-beta, PP2A B subunit isoform R5-beta, PPP2R5B

1 Images
Western blot - Anti-PPP2R5B antibody (AB1366)
  • WB

Unknown

Western blot - Anti-PPP2R5B antibody (AB1366)

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Western blot - Anti-PPP2R5B antibody (ab1366) at 2 µg/mL

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Human cerebellum lysate at 35 µg

Predicted band size: 57 kDa

Observed band size: 55 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15173

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP2R5B also known as Protein Phosphatase 2 Regulatory Subunit B56 Beta is a regulatory subunit of protein phosphatase 2A (PP2A). It has a molecular mass of approximately 56 kDa. This protein is expressed in various tissues with higher expression in the brain heart and skeletal muscle. Its mechanical function involves modulating PP2A activity by directing the phosphatase to specific substrates which impacts diverse cellular processes.
Biological function summary

The subunit modulates several key processes including cell growth division and apoptosis. It serves as part of the PP2A holoenzyme complex a major Ser/Thr phosphatase. This complex influences many signaling pathways by dephosphorylating target proteins. Through interactions with other B subunits the specific regulatory effects of B56 beta become fine-tuned providing this subunit with precise control over cellular outcomes.

Pathways

PPP2R5B plays a role in essential signaling cascades such as the MAPK/ERK and PI3K/AKT pathways. These pathways regulate diverse cell functions including proliferation and survival. PPP2R5B coordinates with other proteins like Bcl-2 and p53 in these pathways to regulate apoptosis and cell cycle progression. Its ability to influence these pathways places it at a pivotal point in the signal transduction network.

Alterations in PPP2R5B function have links to cancer development and Alzheimer’s disease. In cancer its relationship with p53 suggests a role in tumor suppression as PPP2R5B can affect p53 stability. In neurodegenerative contexts such as Alzheimer’s the improper regulation of phosphorylation sites could contribute to pathogenesis again implicating pathways like PI3K/AKT. Identifying PPP2R5B interactions is important for understanding disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

As the regulatory component of the serine/threonine-protein phosphatase 2A (PP2A) holoenzyme, modulates substrate specificity, subcellular localization, and responsiveness to phosphorylation. The phosphorylated form mediates the interaction between PP2A and AKT1, leading to AKT1 dephosphorylation.
See full target information Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 288:1032-46 PubMed23204520

2012

Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart.

Applications

WB, WB, WB, WB

Species

Rat, Mouse, Dog, Human

Sean T DeGrande,Sean C Little,Derek J Nixon,Patrick Wright,Jedidiah Snyder,Wen Dun,Nathaniel Murphy,Ahmet Kilic,Robert Higgins,Philip F Binkley,Penelope A Boyden,Cynthia A Carnes,Mark E Anderson,Thomas J Hund,Peter J Mohler
View all publications
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Product promise

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