Rabbit Polyclonal PPP2R5C antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PPP2R5C aa 450 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PPP2R5C aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13362
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Target data
Function
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. The PP2A-PPP2R5C holoenzyme may specifically dephosphorylate and activate TP53 and play a role in DNA damage-induced inhibition of cell proliferation. PP2A-PPP2R5C may also regulate the ERK signaling pathway through ERK dephosphorylation.
Alternative names
KIAA0044, PPP2R5C, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform, PP2A B subunit isoform B'-gamma, PP2A B subunit isoform B56-gamma, PP2A B subunit isoform PR61-gamma, PP2A B subunit isoform R5-gamma, Renal carcinoma antigen NY-REN-29
Recommended products
Rabbit Polyclonal PPP2R5C antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PPP2R5C aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PPP2R5C aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q13362
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein phosphatase 2 regulatory subunit B' gamma also known as PPP2R5C is a regulatory component of the protein phosphatase 2A (PP2A) holoenzyme. PPP2R5C has a molecular weight of approximately 59 kDa. This protein shows expression in various tissues including brain kidney spleen and liver. PPP2R5C serves as a modulator by altering the substrate selectivity or catalytic activity of the PP2A core enzyme which consists of a catalytic subunit and a structural subunit.
- Biological function summary
The protein plays a role in mediating cellular processes through its participation as part of the PP2A holoenzyme complex. PP2A regulates various cellular activities including cell cycle progression apoptosis and signal transduction. PPP2R5C contributes to these biological processes by modulating enzyme activity and directing the PP2A holoenzyme to specific subcellular compartments or substrates. This makes PPP2R5C essential for maintaining cellular homeostasis and response to environmental signals.
- Pathways
PPP2R5C functions in key signaling pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. In the MAPK/ERK pathway PPP2R5C affects phosphorylation states and downstream signaling outcomes. The protein also interacts with other components such as AKT enabling regulation of cellular growth metabolism and survival mechanisms. These interactions define the influence of PPP2R5C on signal propagation and impact the overall cellular response.
- Associated diseases and disorders
Alterations in PPP2R5C have been implicated in cancer and neurodegenerative diseases. In cancer abnormal PP2A activity linked with PPP2R5C affects cell cycle regulation and tumorigenesis interacting with cancer-related pathways and proteins like MYC. In neurodegenerative diseases dysfunctions in signaling mechanisms where PPP2R5C is involved contribute to disease progression affecting proteins related to neuron survival and function. Understanding the role of PPP2R5C in these diseases provides insight into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-PPP2R5C antibody (ab264338)
All lanes: Western blot - Anti-PPP2R5C antibody (ab264338) at 0.04 µg/mL
Lane 1: HEK-293T whole cell lysate at 50 µg
Lane 2: HEK-293T whole cell lysate at 15 µg
Lane 3: HeLa whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Exposure time: 3min
Immunoprecipitation - Anti-PPP2R5C antibody (ab264338)
PPP2R5C was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264338 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab264338 at 1 μg/ml.
Lane 1: ab264338 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 30 secs.
All lanes: Immunoprecipitation - Anti-PPP2R5C antibody (ab264338)
Predicted band size: 61 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP2R5C antibody (ab264338)
Immunohistochemical analysis of FFPE human breast carcinoma tissue section labeling PPP2R5C with ab264338 at 1/1000 dilution. Hematoxylin was used as counterstain. Detection: DAB.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
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