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AB316368

Anti-PPP2R5D antibody [H5D12]

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Mouse Monoclonal PPP2R5D antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform.

View Alternative Names

Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform, PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform PR61-delta, PP2A B subunit isoform R5-delta, PPP2R5D

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5D Antibody [H5D12] (AB316368)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R5D Antibody [H5D12] (AB316368)

Immunofluorescence analysis of paraformaldehyde fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Western blot - Anti-PPP2R5D Antibody [H5D12] (AB316368)
  • WB

Supplier Data

Western blot - Anti-PPP2R5D Antibody [H5D12] (AB316368)

(1 µg/ml) staining in HeLa (A), (0.1 µg/ml) staining in MCF7 (B), A431 (C) and (0.03 µg/ml) staining in Jurkat (D) cells lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Lane 1:

Western blot - Anti-PPP2R5D Antibody [H5D12] (ab316368) at 1 µg/mL

Lane 1:

HeLa cell lysate

Lanes 2 - 3:

Western blot - Anti-PPP2R5D Antibody [H5D12] (ab316368) at 0.1 µg/mL

Lane 2:

MCF7 cell lysate

Lane 3:

A431 cell lysate

Lane 4:

Western blot - Anti-PPP2R5D Antibody [H5D12] (ab316368) at 0.03 µg/mL

Lane 4:

Jurkat cell lysate

true

Exposure time: 1hr

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

H5-D12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform.

Q14738

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Proteins & Peptides

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1 month
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP2R5D also known as B56δ forms part of the regulatory subunit of protein phosphatase 2A (PP2A). Protein phosphatase 2A is an important enzyme in cellular signaling that dephosphorylates target proteins. The approximate molecular mass of PPP2R5D is 72 kDa. It is found expressed in various tissues throughout the human body but its levels can vary between different cell types.
Biological function summary

PPP2R5D plays an essential role in the regulation of cell growth and division. This protein is part of the PP2A holoenzyme complex which assembles with catalytic and scaffolding subunits. Through its regulatory influence PPP2R5D affects the phosphorylation state of several substrates thereby impacting cellular processes like cell cycle progression and apoptosis.

Pathways

PPP2R5D significantly contributes to the MAPK signaling pathway and the Wnt pathway. It modulates phosphorylation states affecting signal transduction mechanisms. In the MAPK pathway PPP2R5D interacts with proteins like ERK1/2 by influencing their activity. Similarly in the Wnt pathway it acts on components important for maintaining cellular balance and signaling fidelity.

PPP2R5D mutations and dysregulation have been linked to intellectual disabilities and cancer. Changes in its function are associated with Autism Spectrum Disorder where it potentially interacts with other proteins involved in neuronal development. In cancer altered PPP2R5D activity may disrupt normal cellular growth control often involving proteins like c-Myc that are critical for proliferation and survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and might also direct the localization of the catalytic enzyme to a particular subcellular compartment.
See full target information Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform
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Product promise

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For full details, please see our Terms & Conditions

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