Rabbit Polyclonal PPP3CB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3CB aa 1-250.
Images
Publications
Filter by
- Nature communications 8:22702017Small-molecule TFEB pathway agonists that ameliorate metabolic syndrome in mice and extend C. elegans lifespan.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChensu Wang et. al.PubMed 29273768
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PPP3CB aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16298
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 7.5 µg/mL | Notes Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:19154138, PubMed:25720963, PubMed:26794871, PubMed:32753672). Dephosphorylates TFEB in response to lysosomal Ca(2+) release, resulting in TFEB nuclear translocation and stimulation of lysosomal biogenesis (PubMed:25720963, PubMed:32753672). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). May play a role in skeletal muscle fiber type specification (By similarity).
Alternative names
CALNA2, CALNB, CNA2, PPP3CB, Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit beta isoform, CNA beta
Recommended products
Rabbit Polyclonal PPP3CB antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3CB aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PPP3CB aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P16298
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PPP3CB also known as Calcineurin subunit B type 2 plays an important role in cellular signal transduction. It is a catalytic subunit of the protein phosphatase 3 which requires calcium ions and calmodulin to become activated. PPP3CB is expressed in many tissues with significant levels in the brain and immune cells. Its molecular mass is approximately 59.6 kDa. The protein’s primary mechanical function involves dephosphorylation of serine-threonine residues in proteins which affects various cellular processes.
- Biological function summary
PPP3CB is essential in neuronal and immune system function. It is a component of the calcineurin complex composed of a catalytic A subunit and a regulatory B subunit. In neurons it regulates synaptic plasticity and memory functions by modulating neurotransmitter responses. In the immune system it controls T-cell activation and differentiation by mediating the nuclear factor of activated T-cells (NFAT) signaling pathway which is important for the transcription of interleukin-2 and other cytokines.
- Pathways
PPP3CB has major involvement in both the calcium signaling and immune response pathways. In the calcium signaling pathway its interaction with calmodulin allows for the activation of downstream signals that influence cellular responses such as growth differentiation and apoptosis. In immune response pathways PPP3CB by controlling NFATc dephosphorylation influences genes critical for immune cell function. It relates closely to proteins like calmodulin and NFAT which play roles in these pathways.
- Associated diseases and disorders
PPP3CB shows a significant link to Alzheimer's disease and autoimmune diseases. Alzheimer's disease involves impaired calcineurin activity which affects synaptic plasticity and memory function. Autoimmune diseases often involve aberrant T-cell activation where PPP3CB plays a modulatory role through NFAT signaling. Dysregulation in this pathway connected to proteins like NFAT can contribute to conditions such as rheumatoid arthritis or systemic lupus erythematosus.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP3CB antibody (ab191374)
Immunohistochemical analysis of formalin fixed paraffin embedded human brain cortex labeling PPP3CB with ab191374 at 7.5 μg/ml
Western blot - Anti-PPP3CB antibody (ab191374)
7.5 % SDS PAGE
All lanes: Western blot - Anti-PPP3CB antibody (ab191374) at 1/5000 dilution
All lanes: Molt-4 cell lysate at 30 µg
Predicted band size: 59 kDa
Western blot - Anti-PPP3CB antibody (ab191374)
7.5% SDS PAGE
All lanes: Western blot - Anti-PPP3CB antibody (ab191374) at 1/1000 dilution
All lanes: Mouse brain lysate at 50 µg
Predicted band size: 59 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com