Rabbit Polyclonal PPP3CB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3CB aa 1-100.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PPP3CB aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P16298
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:19154138, PubMed:25720963, PubMed:26794871, PubMed:32753672). Dephosphorylates TFEB in response to lysosomal Ca(2+) release, resulting in TFEB nuclear translocation and stimulation of lysosomal biogenesis (PubMed:25720963, PubMed:32753672). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). May play a role in skeletal muscle fiber type specification (By similarity).
Alternative names
CALNA2, CALNB, CNA2, PPP3CB, Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit beta isoform, CNA beta
Recommended products
Rabbit Polyclonal PPP3CB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3CB aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PPP3CB aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P16298
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein PPP3CB also known as Calcineurin subunit B type 2 plays an important role in cellular signal transduction. It is a catalytic subunit of the protein phosphatase 3 which requires calcium ions and calmodulin to become activated. PPP3CB is expressed in many tissues with significant levels in the brain and immune cells. Its molecular mass is approximately 59.6 kDa. The protein’s primary mechanical function involves dephosphorylation of serine-threonine residues in proteins which affects various cellular processes.
- Biological function summary
PPP3CB is essential in neuronal and immune system function. It is a component of the calcineurin complex composed of a catalytic A subunit and a regulatory B subunit. In neurons it regulates synaptic plasticity and memory functions by modulating neurotransmitter responses. In the immune system it controls T-cell activation and differentiation by mediating the nuclear factor of activated T-cells (NFAT) signaling pathway which is important for the transcription of interleukin-2 and other cytokines.
- Pathways
PPP3CB has major involvement in both the calcium signaling and immune response pathways. In the calcium signaling pathway its interaction with calmodulin allows for the activation of downstream signals that influence cellular responses such as growth differentiation and apoptosis. In immune response pathways PPP3CB by controlling NFATc dephosphorylation influences genes critical for immune cell function. It relates closely to proteins like calmodulin and NFAT which play roles in these pathways.
- Associated diseases and disorders
PPP3CB shows a significant link to Alzheimer's disease and autoimmune diseases. Alzheimer's disease involves impaired calcineurin activity which affects synaptic plasticity and memory function. Autoimmune diseases often involve aberrant T-cell activation where PPP3CB plays a modulatory role through NFAT signaling. Dysregulation in this pathway connected to proteins like NFAT can contribute to conditions such as rheumatoid arthritis or systemic lupus erythematosus.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-PPP3CB antibody (ab219984)
Immunofluorescent analysis of U251 MG cells (PFA-fixed/Triton X-100 permeabilized) labeling PPP3CB with ab219984 at 4 μg/ml (green).
Western blot - Anti-PPP3CB antibody (ab219984)
All lanes: Western blot - Anti-PPP3CB antibody (ab219984) at 1/100 dilution
All lanes: RT4 cell lysate
Predicted band size: 59 kDa
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