AB219984

Anti-PPP3CB antibody

Name: Anti-PPP3CB antibody (ab219984) | Abcam
Brand: Abcam Limited
SKU: AB219984
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal PPP3CB antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PPP3CB aa 1-100.

View Alternative Names

CALNA2, CALNB, CNA2, PPP3CB, Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit beta isoform, CNA beta

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PPP3CB antibody (AB219984)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PPP3CB antibody (AB219984)

Immunofluorescent analysis of U251 MG cells (PFA-fixed/Triton X-100 permeabilized) labeling PPP3CB with ab219984 at 4 μg/ml (green).

Unknown

Western blot - Anti-PPP3CB antibody (AB219984)

All lanes:

Western blot - Anti-PPP3CB antibody (ab219984) at 1/100 dilution

All lanes:

RT4 cell lysate

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PPP3CB aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P16298

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PPP3CB also known as Calcineurin subunit B type 2 plays an important role in cellular signal transduction. It is a catalytic subunit of the protein phosphatase 3 which requires calcium ions and calmodulin to become activated. PPP3CB is expressed in many tissues with significant levels in the brain and immune cells. Its molecular mass is approximately 59.6 kDa. The protein’s primary mechanical function involves dephosphorylation of serine-threonine residues in proteins which affects various cellular processes.

Biological function summary

PPP3CB is essential in neuronal and immune system function. It is a component of the calcineurin complex composed of a catalytic A subunit and a regulatory B subunit. In neurons it regulates synaptic plasticity and memory functions by modulating neurotransmitter responses. In the immune system it controls T-cell activation and differentiation by mediating the nuclear factor of activated T-cells (NFAT) signaling pathway which is important for the transcription of interleukin-2 and other cytokines.

Pathways

PPP3CB has major involvement in both the calcium signaling and immune response pathways. In the calcium signaling pathway its interaction with calmodulin allows for the activation of downstream signals that influence cellular responses such as growth differentiation and apoptosis. In immune response pathways PPP3CB by controlling NFATc dephosphorylation influences genes critical for immune cell function. It relates closely to proteins like calmodulin and NFAT which play roles in these pathways.

PPP3CB shows a significant link to Alzheimer's disease and autoimmune diseases. Alzheimer's disease involves impaired calcineurin activity which affects synaptic plasticity and memory function. Autoimmune diseases often involve aberrant T-cell activation where PPP3CB plays a modulatory role through NFAT signaling. Dysregulation in this pathway connected to proteins like NFAT can contribute to conditions such as rheumatoid arthritis or systemic lupus erythematosus.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed : 19154138, PubMed : 25720963, PubMed : 26794871, PubMed : 32753672). Dephosphorylates TFEB in response to lysosomal Ca(2+) release, resulting in TFEB nuclear translocation and stimulation of lysosomal biogenesis (PubMed : 25720963, PubMed : 32753672). Dephosphorylates and activates transcription factor NFATC1 (PubMed : 19154138). Dephosphorylates and inactivates transcription factor ELK1 (PubMed : 19154138). Dephosphorylates DARPP32 (PubMed : 19154138). Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). May play a role in skeletal muscle fiber type specification (By similarity).

See full target information PPP3CB

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 16:1629-1646 PubMed33495627

2021

Nuclei isolation of multiple brain cell types for omics interrogation.

Applications

Unspecified application

Species

Unspecified reactive species

Alexi Nott,Johannes C M Schlachetzki,Bethany R Fixsen,Christopher K Glass

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PPP3CB antibody

Immunocytochemistry/ Immunofluorescence - Anti-PPP3CB antibody (AB219984)

Western blot - Anti-PPP3CB antibody (AB219984)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise