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AB249162

Anti-PPP3CC antibody [EPR10601] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPP3CC antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CALNA3, CNA3, PPP3CC, Serine/threonine-protein phosphatase 2B catalytic subunit gamma isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit gamma isoform

1 Images
Western blot - Anti-PPP3CC antibody [EPR10601] - BSA and Azide free (AB249162)
  • WB

Unknown

Western blot - Anti-PPP3CC antibody [EPR10601] - BSA and Azide free (AB249162)

This data was developed using ab154863, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPP3CC antibody [EPR10601] (<a href='/en-us/products/primary-antibodies/ppp3cc-antibody-epr10601-ab154863'>ab154863</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10601

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249162 is the carrier-free version of ab154863.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PPP3CC gene encodes the catalytic subunit of the protein phosphatase 3 also known as calcineurin gamma which weighs approximately 58 kDa. This enzyme acts as a calcium/calmodulin-dependent serine/threonine phosphatase that modulates various cellular functions by dephosphorylating target proteins. It is widely expressed across various tissues but its presence is significant in the brain where it plays an important part in neural signaling and synaptic plasticity.
Biological function summary

The protein phosphatase 3 complex involves PPP3CC which contributes to numerous cellular signaling pathways by regulating intracellular levels of calcium. This regulation impacts synaptic transmission neuronal excitability and immune responses. In the neuronal context it helps modulate pathways associated with long-term potentiation and learning by influencing the recruitment and function of neurotransmitter receptors.

Pathways

PPP3CC contributes a regulatory role to the calcium signaling pathway and the calcineurin-NFAT pathway. In these pathways it interacts with and dephosphorylates the NFAT transcription factors influencing gene transcription related to immune response and neural activities. Through these interactions it shares functional relationships with other proteins like NFAT and calmodulin which also participate in these pathways.

Irregular activity of PPP3CC links to neurological disorders such as Alzheimer’s disease and schizophrenia. Altered calcineurin signaling involving the PPP3CC subunit associates with disrupted synaptic functions and neuronal communication in these conditions. Furthermore in Alzheimer's disease the dysregulation may involve other proteins like tau or amyloid precursor protein influencing disease progression and severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals. Dephosphorylates and activates transcription factor NFATC1. Dephosphorylates and inactivates transcription factor ELK1. Dephosphorylates DARPP32.
See full target information PPP3CC
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com