AB70631

Anti-PPP4R2 antibody

Name: Anti-PPP4R2 antibody (ab70631) | Abcam
Brand: Abcam Limited
SKU: AB70631
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PPP4R2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PPP4R2.

View Alternative Names

SBBI57, PPP4R2, Serine/threonine-protein phosphatase 4 regulatory subunit 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP4R2 antibody (AB70631)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP4R2 antibody (AB70631)

Immunohistochemical analysis of human ovarian cancer tissue, labelling PPP4R2 with ab70631 at 1/200 dilution.

Unknown

Immunoprecipitation - Anti-PPP4R2 antibody (AB70631)

Detection of Human PPP4R2 by Western Blot of Immunprecipitate. ab70631 at 1µg/ml staining PPP4R2 in HeLa whole cell lysate immunoprecipitated using ab70631 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PPP4R2 antibody (ab70631)

Predicted band size: 47 kDa

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Unknown

Western blot - Anti-PPP4R2 antibody (AB70631)

All lanes:

Western blot - Anti-PPP4R2 antibody (ab70631) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HEK293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human PPP4R2. The exact immunogen used to generate this antibody is proprietary information.

Q9NY27

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase 4 regulatory subunit 2 commonly known as PPP4R2 serves as a regulatory subunit in the protein phosphatase 4 (PP4) complex. It has an approximate mass of 50 kDa. PPP4R2 is mainly expressed in the cytoplasm of cells where it regulates the activity and specificity of PP4. It is involved in various cellular processes due to its capacity to modulate PP4's activity.

Biological function summary

PPP4R2 plays a significant role in DNA damage repair processes. PPP4R2 is part of the PP4 complex and together with other components it oversees dephosphorylation processes post DNA damage which is essential for cell cycle progression and genomic stability. It collaborates with other proteins in the complex to ensure accurate DNA repair maintaining proper cellular function and preventing mutations.

Pathways

PPP4R2 is notably involved in the DNA repair pathway and cell cycle regulation pathway. These pathways are critical for maintaining cell integrity and viability. Within the DNA repair pathway PPP4R2 operates closely with proteins like PP4C the catalytic subunit of PP4 which performs dephosphorylation tasks essential for DNA damage response. In the cell cycle regulation pathway PPP4R2 ensures appropriate cell cycle checkpoints by interacting with multiple cellular proteins which helps in maintaining genomic stability.

Research links PPP4R2 to cancer and neurodegenerative diseases highlighting its importance in maintaining genomic integrity. In cancer alterations in PPP4R2 expression or function can affect DNA repair leading to genomic instability and tumorigenesis. PPP4R2 interacts with proteins such as CHK2 that are important in the DNA damage response. Moreover abnormal PPP4R2 activity impacts neurodegenerative conditions where defects in DNA repair mechanisms are implicated. Understanding its role in these contexts aids in potential therapeutic targeting strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair.

See full target information PPP4R2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:52255-52269 PubMed27391343

2016

PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-κB signaling in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yi Ho,Chi-Ming Liang,Shu-Mei Liang

The Journal of biological chemistry 283:29273-84 PubMed18715871

2008

PP4R4/KIAA1622 forms a novel stable cytosolic complex with phosphoprotein phosphatase 4.

Applications

Unspecified application

Species

Unspecified reactive species

Ginny I Chen,Sally Tisayakorn,Claus Jorgensen,Lisa M D'Ambrosio,Marilyn Goudreault,Anne-Claude Gingras

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PPP4R2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPP4R2 antibody (AB70631)

Immunoprecipitation - Anti-PPP4R2 antibody (AB70631)

Western blot - Anti-PPP4R2 antibody (AB70631)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise