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AB70634

Anti-PPP6C/Ppv antibody

4

(1 Review)

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(1 Publication)

Rabbit Polyclonal PPP6C/Ppv antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PPP6C.

View Alternative Names

PPP6, PPP6C, Serine/threonine-protein phosphatase 6 catalytic subunit, PP6C

2 Images
Immunoprecipitation - Anti-PPP6C/Ppv antibody (AB70634)
  • IP

Unknown

Immunoprecipitation - Anti-PPP6C/Ppv antibody (AB70634)

Detection of PPP6C/Ppv by Western Blot of Immunoprecipitate. ab70634 at 1μg/ml staining PPP6C/Ppv in HeLa whole cell lysate immunoprecipitated using ab70634 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PPP6C/Ppv antibody (ab70634)

Predicted band size: 35 kDa

false

Western blot - Anti-PPP6C/Ppv antibody (AB70634)
  • WB

Unknown

Western blot - Anti-PPP6C/Ppv antibody (AB70634)

All lanes:

Western blot - Anti-PPP6C/Ppv antibody (ab70634) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 35 kDa

Observed band size: 35 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PPP6C. The exact immunogen used to generate this antibody is proprietary information.

O00743

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP6C also known as Protein Phosphatase 6 Catalytic Subunit is a protein phosphatase which participates in dephosphorylation processes. It has a molecular mass of approximately 35 kDa. PPP6C locates mainly in the cytoplasm but has also been found in the nucleus. Its expression is widespread across various tissues including the brain heart and skeletal muscle. PPP6C is important in controlling cellular functions by removing phosphate groups from target molecules influencing their activity state.
Biological function summary

The protein influences cell cycle regulation cell proliferation and apoptosis. This protein typically functions as part of a larger enzymatic complex that includes regulatory and scaffold subunits. By associating with these proteins PPP6C gains specificity towards its substrates and ensures precise cellular responses. Its activity helps maintain cellular homeostasis and proper cell growth and it also modulates immune response pathways by controlling phosphatase activity which is especially critical for immune system functioning.

Pathways

PPP6C interacts with key cellular signaling pathways such as the NF-kB and AKT pathways. During the regulation of these pathways PPP6C associates with proteins like IKK and PDK1 respectively which are important in mediating responses to external stimuli and regulating cell survival. Inhibition or dysregulation of PPP6C can impact these pathways leading to aberrant signaling and altered cellular outcomes. PPP6C's influence on these pathways highlights its role in fine-tuning cellular responses to diverse signals.

PPP6C has connections to melanoma and multiple myeloma. Mutations affecting PPP6C may lead to abnormal protein function contributing to the development of these cancers. For example in melanoma alterations in PPP6C can disrupt cellular signaling pathways often in coordination with related proteins like BRAF and NRAS. Understanding these interactions and their effects on disease progression can provide insights into cancer therapeutics and the development of targeted treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of protein phosphatase 6 (PP6) (PubMed : 17079228, PubMed : 29053956, PubMed : 32474700). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation (PubMed : 10227379). N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1 (PubMed : 17568194). During mitosis, regulates spindle positioning (PubMed : 27335426). Down-regulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7 (PubMed : 17079228). Also participates in the innate immune defense against viruses by desphosphorylating RIGI, an essential step that triggers RIGI-mediated signaling activation (PubMed : 29053956). Also regulates innate immunity by acting as a negative regulator of the cGAS-STING pathway : mediates dephosphorylation and inactivation of CGAS and STING1 (PubMed : 32474700, PubMed : 32753499). CGAS dephosphorylation at 'Ser-435' impairs its ability to bind GTP, thereby inactivating it (PubMed : 32474700).
See full target information PPP6C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:27114 PubMed27255161

2016

A protein interaction map for cell-cell adhesion regulators identifies DUSP23 as a novel phosphatase for β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Leon Gallegos,Mei Rosa Ng,Mathew E Sowa,Laura M Selfors,Anne White,Ioannis K Zervantonakis,Pragya Singh,Sabin Dhakal,J Wade Harper,Joan S Brugge
View all publications
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