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AB248413

Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPP6C/Ppv antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

PPP6, PPP6C, Serine/threonine-protein phosphatase 6 catalytic subunit, PP6C

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)

This data was developed using ab131335, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HeLa cells labelling PPP6C/Ppv with ab131335 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)

This data was developed using ab131335, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa cells labelling PPP6C/Ppv with purified ab131335 at a dilution of 1/280 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Flour® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

Western blot - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)
  • WB

Unknown

Western blot - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)

This data was developed using ab131335, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPP6C/Ppv antibody [EPR8764] (<a href='/en-us/products/primary-antibodies/ppp6c-ppv-antibody-epr8764-ab131335'>ab131335</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Fetal thymus tissue lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 35 kDa

false

OI-RD Scanning - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PPP6C/Ppv antibody [EPR8764] - BSA and Azide free (AB248413)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8764

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab248413 is the carrier-free version of ab131335.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPP6C also known as Protein Phosphatase 6 Catalytic Subunit is a protein phosphatase which participates in dephosphorylation processes. It has a molecular mass of approximately 35 kDa. PPP6C locates mainly in the cytoplasm but has also been found in the nucleus. Its expression is widespread across various tissues including the brain heart and skeletal muscle. PPP6C is important in controlling cellular functions by removing phosphate groups from target molecules influencing their activity state.
Biological function summary

The protein influences cell cycle regulation cell proliferation and apoptosis. This protein typically functions as part of a larger enzymatic complex that includes regulatory and scaffold subunits. By associating with these proteins PPP6C gains specificity towards its substrates and ensures precise cellular responses. Its activity helps maintain cellular homeostasis and proper cell growth and it also modulates immune response pathways by controlling phosphatase activity which is especially critical for immune system functioning.

Pathways

PPP6C interacts with key cellular signaling pathways such as the NF-kB and AKT pathways. During the regulation of these pathways PPP6C associates with proteins like IKK and PDK1 respectively which are important in mediating responses to external stimuli and regulating cell survival. Inhibition or dysregulation of PPP6C can impact these pathways leading to aberrant signaling and altered cellular outcomes. PPP6C's influence on these pathways highlights its role in fine-tuning cellular responses to diverse signals.

PPP6C has connections to melanoma and multiple myeloma. Mutations affecting PPP6C may lead to abnormal protein function contributing to the development of these cancers. For example in melanoma alterations in PPP6C can disrupt cellular signaling pathways often in coordination with related proteins like BRAF and NRAS. Understanding these interactions and their effects on disease progression can provide insights into cancer therapeutics and the development of targeted treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalytic subunit of protein phosphatase 6 (PP6) (PubMed : 17079228, PubMed : 29053956, PubMed : 32474700). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation (PubMed : 10227379). N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1 (PubMed : 17568194). During mitosis, regulates spindle positioning (PubMed : 27335426). Down-regulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7 (PubMed : 17079228). Also participates in the innate immune defense against viruses by desphosphorylating RIGI, an essential step that triggers RIGI-mediated signaling activation (PubMed : 29053956). Also regulates innate immunity by acting as a negative regulator of the cGAS-STING pathway : mediates dephosphorylation and inactivation of CGAS and STING1 (PubMed : 32474700, PubMed : 32753499). CGAS dephosphorylation at 'Ser-435' impairs its ability to bind GTP, thereby inactivating it (PubMed : 32474700).
See full target information PPP6C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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