Anti-PPT1/PPT antibody [EPR27163-2] (BSA and Azide free)

• IHC

Supplier Data

Immunohistochemistry - Anti-PPT1/PPT antibody [EPR27163-2] (BSA and Azide free) (AB302896)

This data was developed using ab302895, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling PPT1/PPT with ab302895 at 1/5000 (0.092 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human lung cancer. The section was incubated with ab302895 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-PPT1/PPT antibody [EPR27163-2] (BSA and Azide free) (AB302896)

This data was developed using ab302895, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling PPT1/PPT with ab302895 at 1/5000 (0.092 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebrum. The section was incubated with ab302895 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC

Supplier Data

Immunohistochemistry - Anti-PPT1/PPT antibody [EPR27163-2] (BSA and Azide free) (AB302896)

This data was developed using ab302895, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling PPT1/PPT with ab302895 at 1/5000 (0.092 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human cerebellum. The section was incubated with ab302895 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PPT1/PPT antibody [EPR27163-2] (BSA and Azide free) (AB302896)

This data was developed using ab302895, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling PPT1/PPT with ab302895 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

• ICC

Supplier Data

Immunocytochemistry - Anti-PPT1/PPT antibody [EPR27163-2] (BSA and Azide free) (AB302896)

This data was developed using ab302895, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 80% Methanol-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling PPT1/PPT with ab302895 at 1/50 (9.24 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cell line. is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

• WB

Supplier Data

Western blot - Anti-PPT1/PPT antibody [EPR27163-2] (BSA and Azide free) (AB302896)

This data was developed using ab302895, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

Exposure time :

Lane 1 : 15 seconds

Lanes 2-3 : 180 seconds

All lanes:

Western blot - Anti-PPT1/PPT antibody [EPR27163-2] (<a href='/en-us/products/primary-antibodies/ppt1-ppt-antibody-epr27163-2-ab302895'>ab302895</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human hypothalamus tissue lysate at 20 µg

Lane 3:

Human lung cancer tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 34 kDa

Observed band size: 31-35 kDa

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