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AB122548

Anti-PPTC7 antibody

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(2 Publications)

Rabbit Polyclonal PPTC7 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PPTC7 aa 100-250.

View Alternative Names

TAPP2C, PPTC7, Protein phosphatase PTC7 homolog, T-cell activation protein phosphatase 2C, T-cell activation protein phosphatase 2C-like, TA-PP2C

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human liver tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-PPTC7 antibody (AB122548)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PPTC7 antibody (AB122548)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122548 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPTC7 antibody (AB122548)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human rectum tissue labeling PPTC7 with ab122548 at a 1/500 dilution.

Western blot - Anti-PPTC7 antibody (AB122548)
  • WB

Supplier Data

Western blot - Anti-PPTC7 antibody (AB122548)

All lanes:

Western blot - Anti-PPTC7 antibody (ab122548) at 0.4 µg/mL

Lane 1:

RT4 Cell lysate

Lane 2:

U 251 MG Cell lysate

Predicted band size: 33 kDa

false

Western blot - Anti-PPTC7 antibody (AB122548)
  • WB

Supplier Data

Western blot - Anti-PPTC7 antibody (AB122548)

All lanes:

Western blot - Anti-PPTC7 antibody (ab122548) at 0.4 µg/mL

Lane 1:

NIH-3T3 cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PPTC7 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q8NI37

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPTC7 also known as protein phosphatase targeting orey complex 7 functions as a serine/threonine phosphatase. It has a molecular mass of approximately 33 kDa. The protein is expressed in various tissues with high expression in the liver and skeletal muscle. PPTC7 plays a role in mitochondrial protein import dephosphorylating transporter components to facilitate their entry into mitochondria.
Biological function summary

The protein plays a role in mitochondrial health and energy metabolism. By dephosphorylating specific targets it maintains the proper function of mitochondria essential for energy production. The protein is not part of a larger complex but works with components within the mitochondria to support their operations. PPTC7 ensures that the mitochondrial proteins are in the right phosphorylation state which is important for cellular energy needs.

Pathways

PPTC7 interacts with cellular energy metabolic pathways particularly those involving oxidative phosphorylation. The protein interacts with TOM-TIM complexes essential for protein translocation into mitochondria. These interactions affect mitochondrial import regulation ensuring cells efficiently perform energy production processes.

Dysfunction in PPTC7 activity correlates with metabolic disorders and mitochondrial diseases. Aberrant function of this protein can lead to defects in energy metabolism contributing to conditions like mitochondrial myopathy. PPTC7 through its role in mitochondria maintenance influences disorders where metabolism is affected similar to other mitochondrial-involved proteins like PINK1 or MFN2 which are important in neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase that plays an essential role in mitochondrial metabolism and biogenesis (PubMed : 30267671). Positively regulates biosynthesis of the ubiquinone, coenzyme Q (PubMed : 30267671). Dephosphorylates the ubiquinone biosynthesis protein COQ7 which is likely to lead to its activation (PubMed : 30267671). Serves as a crucial sensor for mitophagy, though the underlying mechanism remains ambiguous (By similarity). May dephosphorylate BNIP3 and NIX and thereby directly regulates mitophagy receptor function and stability (By similarity). Alternatively, promotes SCF-FBXL4-dependent ubiquitination and degradation of BNIP3 and NIX independently of its catalytic activity to restrain mitophagy (PubMed : 38992176).
See full target information PPTC7
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 27:1272-1287 PubMed40715440

2025

Reconstitution of BNIP3/NIX-mitophagy initiation reveals hierarchical flexibility of the autophagy machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Elias Adriaenssens,Stefan Schaar,Annan S I Cook,Jan F M Stuke,Justyna Sawa-Makarska,Thanh Ngoc Nguyen,Xuefeng Ren,Martina Schuschnig,Julia Romanov,Grace Khuu,Louise Uoselis,Michael Lazarou,Gerhard Hummer,James H Hurley,Sascha Martens

eLife 6: PubMed29043977

2017

A protein phosphatase network controls the temporal and spatial dynamics of differentiation commitment in human epidermis.

Applications

Unspecified application

Species

Unspecified reactive species

Ajay Mishra,Bénédicte Oulès,Angela Oliveira Pisco,Tony Ly,Kifayathullah Liakath-Ali,Gernot Walko,Priyalakshmi Viswanathan,Matthieu Tihy,Jagdeesh Nijjher,Sara-Jane Dunn,Angus I Lamond,Fiona M Watt
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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