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Rabbit Polyclonal PPWD1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Peptidylprolyl isomerase domain and WD repeat-containing protein 1 aa 1-250.

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Images

Western blot - Anti-PPWD1 antibody (AB154040), expandable thumbnail
  • Western blot - Anti-PPWD1 antibody (AB154040), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PPWD1 antibody (AB154040), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Peptidylprolyl isomerase domain and WD repeat-containing protein 1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BP3

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested
Rat
Tested
Expected

Tested
Tested

Species

Rat

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed:20676357). May be involved in pre-mRNA splicing (PubMed:11991638).

Alternative names

Recommended products

Rabbit Polyclonal PPWD1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Peptidylprolyl isomerase domain and WD repeat-containing protein 1 aa 1-250.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Peptidylprolyl isomerase domain and WD repeat-containing protein 1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BP3
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The protein of interest PPWD1 also known as Peptidylprolyl isomerase domain and WD repeat-containing protein 1 is a part of the cyclophilin family. This protein has a molecular mass of approximately 105 kDa. It is expressed in a variety of tissues including liver and brain. Mechanically PPWD1 functions as a peptidylprolyl isomerase a type of enzyme that catalyzes the cis-trans isomerization of proline residues in peptides and proteins. This action assists in protein folding which is a critical process for proper cellular function.

Biological function summary

PPWD1 plays a fundamental role in the splicing of pre-mRNA by being a part of the spliceosome complex. This complex is essential for the accurate removal of introns from pre-mRNA therefore shaping the final mRNA transcript that gets translated into protein. The WD40 repeat in PPWD1 helps in mediating protein-protein interactions which is vital for its function in the spliceosome complex. This ensures that PPWD1 interacts efficiently with other spliceosomal components facilitating correct splicing.

Pathways

PPWD1 participates in RNA processing pathways particularly focused on mRNA splicing. It acts in the spliceosomal complex with proteins like snRNPs and various other splicing factors regulating the splicing events. PPWD1 is also linked to pathways governing cellular responses to signals due to its role in managing the diversity of protein products derived from alternative splicing events. This protein influences the pathway in coordination with others such as SFRS1 affecting how cells adapt to signaling changes.

Associated diseases and disorders

PPWD1 has associations with cancer and neurodegenerative diseases. It relates to cancer due to its role in alternative splicing which can affect oncogene expression and tumor suppressor genes promoting disease development. In neurodegenerative disorders PPWD1's malfunction may impact neuron-specific gene splicing linking it to diseases like Alzheimer's. Proteins like p53 and tau might interact with splicing regulators including PPWD1 in these contexts underlining its potential involvement in disease pathogenesis.

Product promise

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3 product images

  • Western blot - Anti-PPWD1 antibody (ab154040), expandable thumbnail

    Western blot - Anti-PPWD1 antibody (ab154040)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-PPWD1 antibody (ab154040) at 1/1000 dilution

    All lanes: Rat bone marrow whole cell lysate. at 50 µg

    Predicted band size: 74 kDa

  • Western blot - Anti-PPWD1 antibody (ab154040), expandable thumbnail

    Western blot - Anti-PPWD1 antibody (ab154040)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-PPWD1 antibody (ab154040) at 1/2000 dilution

    All lanes: 293T whole cell lysate at 30 µg

    Predicted band size: 74 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PPWD1 antibody (ab154040), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PPWD1 antibody (ab154040)

    Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling PPWD1 with ab154040 at 1/500 dilution. Alpha-tubulin filaments are labeled red.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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