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AB248140

Anti-PPWD1 antibody [EPR7440] - BSA and Azide free

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Rabbit Recombinant Monoclonal PPWD1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

KIAA0073, PPWD1, Peptidylprolyl isomerase domain and WD repeat-containing protein 1, Spliceosome-associated cyclophilin

2 Images
Western blot - Anti-PPWD1 antibody [EPR7440] - BSA and Azide free (AB248140)
  • WB

Unknown

Western blot - Anti-PPWD1 antibody [EPR7440] - BSA and Azide free (AB248140)

This data was developed using ab126710, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PPWD1 antibody [EPR7440] - BSA and Azide free (ab248140) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

L929 cell lysate at 10 µg

Lane 5:

L6 cell lysate at 10 µg

Lane 6:

C6 cell lysate at 10 µg

Lane 7:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 74 kDa

false

OI-RD Scanning - Anti-PPWD1 antibody [EPR7440] - BSA and Azide free (AB248140)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PPWD1 antibody [EPR7440] - BSA and Azide free (AB248140)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7440

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248140 is the carrier-free version of ab126710.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein of interest PPWD1 also known as Peptidylprolyl isomerase domain and WD repeat-containing protein 1 is a part of the cyclophilin family. This protein has a molecular mass of approximately 105 kDa. It is expressed in a variety of tissues including liver and brain. Mechanically PPWD1 functions as a peptidylprolyl isomerase a type of enzyme that catalyzes the cis-trans isomerization of proline residues in peptides and proteins. This action assists in protein folding which is a critical process for proper cellular function.
Biological function summary

PPWD1 plays a fundamental role in the splicing of pre-mRNA by being a part of the spliceosome complex. This complex is essential for the accurate removal of introns from pre-mRNA therefore shaping the final mRNA transcript that gets translated into protein. The WD40 repeat in PPWD1 helps in mediating protein-protein interactions which is vital for its function in the spliceosome complex. This ensures that PPWD1 interacts efficiently with other spliceosomal components facilitating correct splicing.

Pathways

PPWD1 participates in RNA processing pathways particularly focused on mRNA splicing. It acts in the spliceosomal complex with proteins like snRNPs and various other splicing factors regulating the splicing events. PPWD1 is also linked to pathways governing cellular responses to signals due to its role in managing the diversity of protein products derived from alternative splicing events. This protein influences the pathway in coordination with others such as SFRS1 affecting how cells adapt to signaling changes.

PPWD1 has associations with cancer and neurodegenerative diseases. It relates to cancer due to its role in alternative splicing which can affect oncogene expression and tumor suppressor genes promoting disease development. In neurodegenerative disorders PPWD1's malfunction may impact neuron-specific gene splicing linking it to diseases like Alzheimer's. Proteins like p53 and tau might interact with splicing regulators including PPWD1 in these contexts underlining its potential involvement in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding (PubMed : 20676357). May be involved in pre-mRNA splicing (PubMed : 11991638).
See full target information PPWD1
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Product promise

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For full details, please see our Terms & Conditions

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