AB103632

Anti-PR3 antibody

Name: Anti-PR3 antibody (ab103632) | Abcam
Brand: Abcam Limited
SKU: AB103632
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal PR3 antibody. Carrier free. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRTN3.

View Alternative Names

MBN, PRTN3, Myeloblastin, AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, P29, Wegener autoantigen, PR-3, PR3, NP-4

3 Images

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Western blot - Anti-PR3 antibody (AB103632)

All lanes:

Western blot - Anti-PR3 antibody (ab103632) at 5 µg/mL

All lanes:

Mouse brain tissue lysate at 50 µg

Predicted band size: 27 kDa

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Western blot - Anti-PR3 antibody (AB103632)

All lanes:

Western blot - Anti-PR3 antibody (ab103632) at 5 µg/mL

Lane 1:

PR3 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 27 kDa

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CiteAb

Western blot - Anti-PR3 antibody (AB103632)

PR3 western blot using anti-PR3 antibody ab103632. Publication image and figure legend from Svendsen, J. B., Baslund, B., et al., 2016, PLoS One, PubMed 27755585.

ab103632 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab103632 please see the product overview.

Expression of proteinase 3 in PMNs.(A) Western blot of total PMN protein from 6 healthy controls (HC) and 6 patients with granulomatosis with polyangiitis (GPA). (B) Relative intensities of proteinase 3 following normalization to the corresponding β-actin intensity for the 6 HCs and 6 GPA patients. Horizontal lines indicate medians, and error bars depict interquartile ranges.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRTN3.

P24158

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.

Biological function summary

Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.

Pathways

Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.

PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed : 2033050, PubMed : 28240246, PubMed : 3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed : 23202369). May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed : 22266279).

See full target information PRTN3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1534078 PubMed40028338

2025

Plasma anti-PRTN3 IgG and IgM autoantibodies: novel biomarkers for early detection of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yutong Li,Linhong Wang,Fengqi Chen,Rulan Liao,Jing Li,Xiaobin Cao,Songyun Ouyang,Liping Dai,Renle Du

Arteriosclerosis, thrombosis, and vascular biology 38:1616-1631 PubMed29853563

2018

Deficiency of FAM3D (Family With Sequence Similarity 3, Member D), A Novel Chemokine, Attenuates Neutrophil Recruitment and Ameliorates Abdominal Aortic Aneurysm Development.

Applications

Unspecified application

Species

Unspecified reactive species

Li He,Yi Fu,Jingna Deng,Yicong Shen,Yingbao Wang,Fang Yu,Nan Xie,Zhongjiang Chen,Tianpei Hong,Xinjian Peng,Qingqing Li,Jing Zhou,Jingyan Han,Ying Wang,Jianzhong Xi,Wei Kong

PloS one 11:e0164985 PubMed27755585

2016

MicroRNA-941 Expression in Polymorphonuclear Granulocytes Is Not Related to Granulomatosis with Polyangiitis.

Applications

Species

Human

Jesper Brink Svendsen,Bo Baslund,Elisabeth Præstekjær Cramer,Nicolas Rapin,Niels Borregaard,Jack Bernard Cowland

The Journal of clinical investigation 124:4539-48 PubMed25244098

2014

Papillon-Lefèvre syndrome patient reveals species-dependent requirements for neutrophil defenses.

Applications

Species

Human

Ole E Sørensen,Stine N Clemmensen,Sara L Dahl,Ole Østergaard,Niels H Heegaard,Andreas Glenthøj,Finn Cilius Nielsen,Niels Borregaard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PR3 antibody

Western blot - Anti-PR3 antibody (AB103632)

Western blot - Anti-PR3 antibody (AB103632)

Western blot - Anti-PR3 antibody (AB103632)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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