Anti-PR3 antibody [EPR23253-35] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human spleen.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

ab270441 staining PR3 in HL-60 cells, with negative expression in K562 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab270441 at 5 μg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse spleen. The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse bone marrow or EL4 cells labelling PR3 with ab270441 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in a subset of mouse bone marrow cells. Negative control : EL4 (PMID : 9211743). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human lung (PMID : 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in human placenta.The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling PR3 with ab270441 at 1/100 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on human bone marrow (PMID : 22247758).The section was incubated with ab270441 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PR3 antibody [EPR23253-35] - BSA and Azide free (AB270610)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling PR3 with ab270441 at 1/2000 dilution followed by a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used. Positive staining on neutrophils in mouse lung (PMID : 22247758).The section was incubated with ab270441 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™, Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270441).