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AB91181

Anti-PR3 antibody [WGM2]

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(14 Publications)

Mouse Monoclonal PR3 antibody. Suitable for Flow Cyt, ELISA, WB, IHC-P, FuncS (Neut/Block), IHC-Fr and reacts with Human samples. Cited in 14 publications.

View Alternative Names

MBN, PRTN3, Myeloblastin, AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, P29, Wegener autoantigen, PR-3, PR3, NP-4

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

WGM2

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ELISA, WB, Flow Cyt, IHC-Fr, IHC-P, FuncS (Neut/Block)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product details

ab91181 blocks the PR3 activity and partially inhibits the binding of Human PR3-ANCA to PR3; it can be used in immuno assays, for inhibition of biological PR3 activity and induction of monocytes and neutrophils activation.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
0.2 µm filtered antibody solution
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.
Biological function summary

Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.

Pathways

Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.

PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed : 2033050, PubMed : 28240246, PubMed : 3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed : 23202369). Plays a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed : 22266279). Triggers inflammatory processes in neutrophils by interacting with ADGRG3 upstream of F2RL1/PAR2 activation (PubMed : 36302784).
See full target information PRTN3

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 74:103231 PubMed38861835

2024

Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yu,Yu Fu,Jiameng Gao,Qingqing Zhang,Nan Zhang,Zhiyuan Zhang,Xuemei Jiang,Chang Chen,Zongmei Wen

Oncoimmunology 11:2052418 PubMed35309732

2022

Neutrophil extracellular traps induce tumor metastasis through dual effects on cancer and endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ze-Zhou Jiang,Zhi-Peng Peng,Xing-Chen Liu,Hao-Fan Guo,Meng-Meng Zhou,Da Jiang,Wan-Ru Ning,Yu-Fan Huang,Limin Zheng,Yan Wu

BMC immunology 20:22 PubMed31248381

2019

Macrophage migration inhibitory factor is involved in antineutrophil cytoplasmic antibody-mediated activation of C5a-primed neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hao,Tiegang Lv,Liping Xu,Mao Ran,Kaili Wu

Journal of the American Chemical Society 139:10115-10125 PubMed28672107

2017

Toolbox of Fluorescent Probes for Parallel Imaging Reveals Uneven Location of Serine Proteases in Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Paulina Kasperkiewicz,Yoav Altman,Maximiliano D'Angelo,Guy S Salvesen,Marcin Drag

Disease models & mechanisms 9:885-97 PubMed27483354

2016

Alterations in nuclear structure promote lupus autoimmunity in a mouse model.

Applications

Unspecified application

Species

Human

Namrata Singh,Duncan B Johnstone,Kayla A Martin,Italo Tempera,Mariana J Kaplan,Michael F Denny

PloS one 9:e110677 PubMed25330301

2014

Vesicular LL-37 contributes to inflammation of the lesional skin of palmoplantar pustulosis.

Applications

Unspecified application

Species

Unspecified reactive species

Masamoto Murakami,Takaaki Kaneko,Teruaki Nakatsuji,Kenji Kameda,Hidenori Okazaki,Xiuju Dai,Yasushi Hanakawa,Mikiko Tohyama,Akemi Ishida-Yamamoto,Koji Sayama

Arthritis research & therapy 16:R142 PubMed25000985

2014

The interaction between C5a and sphingosine-1-phosphate in neutrophils for antineutrophil cytoplasmic antibody mediated activation.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Hao,Yi-Min Huang,Ming-Hui Zhao,Min Chen

Innate immunity 20:440-8 PubMed24189124

2013

Myeloperoxidase influences the complement regulatory function of modified C-reactive protein.

Applications

Unspecified application

Species

Human

Peng-cheng Xu,Zhi-ying Li,Xiao-wei Yang,Ming-hui Zhao,Min Chen

Journal of immunology (Baltimore, Md. : 1950) 189:5476-84 PubMed23105141

2012

Broad cross-presentation of the hematopoietically derived PR1 antigen on solid tumors leads to susceptibility to PR1-targeted immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Gheath Alatrash,Elizabeth A Mittendorf,Anna Sergeeva,Pariya Sukhumalchandra,Na Qiao,Mao Zhang,Lisa S St John,Kathryn Ruisaard,Christine E Haugen,Zein Al-Atrache,Haroon Jakher,Anne V Philips,Xiaoling Ding,Jie Qing Chen,Yun Wu,Rebecca S Patenia,Chantale Bernatchez,Luis M Vence,Laszlo G Radvanyi,Patrick Hwu,Karen Clise-Dwyer,Qing Ma,Sijie Lu,Jeffrey J Molldrem

PloS one 7:e38317 PubMed22675451

2012

p38MAPK, ERK and PI3K signaling pathways are involved in C5a-primed neutrophils for ANCA-mediated activation.

Applications

Flow Cyt

Species

Human

Jian Hao,Li-Qiang Meng,Peng-Cheng Xu,Min Chen,Ming-Hui Zhao
View all publications

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