Rabbit Polyclonal PRAK/MK5 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MAPKAPK5 aa 400 to C-terminus.
View Alternative Names
PRAK, MAPKAPK5, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5, p38-regulated/activated protein kinase
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAK/MK5 antibody (AB93800)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PRAK with ab93800 at 1/200 (1µg/ml). Detection : DAB.
- IP
Unknown
Immunoprecipitation - Anti-PRAK/MK5 antibody (AB93800)
Detection of PRAK/MK5 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93800 at 3 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 10 seconds.
All lanes:
Immunoprecipitation - Anti-PRAK/MK5 antibody (ab93800)
Predicted band size: 54 kDa
false
- WB
Unknown
Western blot - Anti-PRAK/MK5 antibody (AB93800)
All lanes:
Western blot - Anti-PRAK/MK5 antibody (ab93800) at 0.04 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 15 µg
Lane 3:
HeLa whole cell lysate at 5 µg
Lane 4:
293T whole cell lysate at 50 µg
Predicted band size: 54 kDa
true
Exposure time: 3min
Alternative Products
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 antibody [EP1720Y] (AB53277)](https://content.abcam.com/products/images/mapk6-erk3-antibody-ep1720y-ab53277--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112506.jpg)
![Western blot - Anti-MK2 antibody [E341] (AB32567)](https://content.abcam.com/products/images/mk2-antibody-e341-ab32567--western-blot-img137079.gif)
Reactivity data
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PRAK/MK5 is a critical component in the regulation of cellular responses to stress stimuli. It is a participant in the activation loop of the MAPK p38 signaling pathway where it serves to influence the phosphorylation state and activity of downstream effectors. PRAK is not typically known to be part of a large complex but it interacts directly with other kinases to amplify or dampen the stress response signal. This allows cells to adapt to varied stress conditions by modulating gene expression apoptosis and other cell fate decisions.
Pathways
PRAK/MK5 plays essential roles within the p38 MAPK and ERK signaling pathways. It closely associates with p38 MAPK which phosphorylates and activates PRAK enabling it to further disseminate the signal. In these pathways PRAK interplays with proteins such as MAPKAPK-2 and HSP27 facilitating diverse cellular activities like inflammation and differentiation. These signaling networks allow PRAK to serve as a link between external stimuli and internal cellular outcomes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Oncogenesis 12:35 PubMed37407566
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com