AB89097

Anti-PRAME antibody

Name: Anti-PRAME antibody (ab89097) | Abcam
Brand: Abcam Limited
SKU: AB89097
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Mouse Polyclonal PRAME antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRAME.

View Alternative Names

MAPE, OIP4, PRAME, Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, Preferentially expressed antigen of melanoma, OIP-4

2 Images

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Western blot - Anti-PRAME antibody (AB89097)

All lanes:

Western blot - Anti-PRAME antibody (ab89097) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 57 kDa

Observed band size: 65 kDa

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Western blot - Anti-PRAME antibody (AB89097)

All lanes:

Western blot - Anti-PRAME antibody (ab89097) at 1 µg/mL

Lane 1:

PRAME transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Predicted band size: 57 kDa

Observed band size: 60 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PRAME.

P78395

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRAME also known as Preferentially Expressed Antigen in Melanoma is a protein typically weighing around 50 kDa. It is found mostly in testis tissue but also appears in several types of tumors including melanoma and some leukemias. PRAME does not show significant expression in most normal tissues making it a potential target for cancer therapies. Immunohistochemistry (IHC) techniques such as PRAME IHC and PRAME staining often use monoclonal antibodies like mAb EPR20330 to detect PRAME protein presence in various samples.

Biological function summary

PRAME acts as a repressor of retinoic acid signaling a process important for cell differentiation and growth. It does not directly form part of a complex but interacts with components involved in retinoic acid pathways. By binding to retinoic acid receptor complexes PRAME prevents activation of genes involved in cell cycle arrest and apoptosis contributing to unchecked cellular proliferation seen in certain cancers.

Pathways

PRAME participates in the retinoic acid and various oncogenic signaling processes. It influences the retinoic acid pathway by interfering with the retinoic acid receptor (RAR) signaling. This interference with key proteins like RAR disrupts the normal regulatory processes that typically inhibit cancer progression. PRAME's modulation of these pathways highlights its role in promoting tumor growth and survival.

PRAME has significant relevance to cancer particularly melanoma. It is often used as a biomarker for melanoma identification due to its overexpression in such tumors. Additionally PRAME's connection to acute myeloid leukemia (AML) emerges from its ability to act similarly in diverse malignant cells. In these diseases the overexpression of PRAME interferes with normal differentiation processes and allows for sustained proliferation of malignant cells suggesting its potential role as a therapeutic target or diagnostic marker in oncology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by gene PRAME functions as a transcriptional repressor, inhibiting retinoic acid signaling through retinoic acid receptors RARA, RARB, and RARG. It prevents retinoic acid-induced cell proliferation arrest, differentiation, and apoptosis. This supplementary information is collated from multiple sources and compiled automatically.

See full target information PRAME

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The American Journal of dermatopathology 44:575-580 PubMed35503885

2022

PRAME Expression Correlates With Genomic Aberration and Malignant Diagnosis of Spitzoid Melanocytic Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Pedram Gerami,Sarah Benton,Jeffrey Zhao,Bin Zhang,Nathaniel Lampley,Andrew Roth,Anastasiya Boutko,Shantel Olivares,Klaus J Busam

International journal of molecular sciences 22: PubMed33804612

2021

Development of a New Highly Selective Monoclonal Antibody against Preferentially Expressed Antigen in Melanoma (PRAME) and Identification of the Target Epitope by Bio-Layer Interferometry.

Applications

Unspecified application

Species

Unspecified reactive species

Jwala Priyadarsini Sivaccumar,Antonio Leonardi,Emanuela Iaccarino,Giusy Corvino,Luca Sanguigno,Angela Chambery,Rosita Russo,Mariangela Valletta,Debora Latino,Domenica Capasso,Nunzianna Doti,Menotti Ruvo,Annamaria Sandomenico

British journal of cancer 115:454-64 PubMed27441500

2016

The cancer/testis-antigen PRAME supports the pluripotency network and represses somatic and germ cell differentiation programs in seminomas.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Nettersheim,Isabell Arndt,Rakesh Sharma,Stefanie Riesenberg,Sina Jostes,Simon Schneider,Michael Hölzel,Glen Kristiansen,Hubert Schorle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PRAME antibody

Western blot - Anti-PRAME antibody (AB89097)

Western blot - Anti-PRAME antibody (AB89097)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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