AB327117
Mouse Monoclonal PRAME antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PRAME.
View Alternative Names
MAPE, OIP4, PRAME, Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, Preferentially expressed antigen of melanoma, OIP-4
1 Images
![Immunocytochemistry/ Immunofluorescence - Anti-PRAME Antibody [CL5146] (AB327117)](https://content.abcam.com/products/images/prame-antibody-cl5146-ab327117--immunocytochemistry-immunofluorescence-img461886.jpg/_jcr_content/renditions/webp-768.webp)
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PRAME Antibody [CL5146] (AB327117)
Immunofluorescence staining of U-2 OS cells using ab327117 at 2–10 µg/ml, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
Properties and storage information
Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Product protocols
For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data
Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation (PubMed : 21822215, PubMed : 26138980). The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding (PubMed : 26138980). In the nucleus, the CRL2(PRAME) complex is recruited to epigenetically and transcriptionally active promoter regions bound by nuclear transcription factor Y (NFY) and probably plays a role in chromstin regulation (PubMed : 21822215). Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG : prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis (PubMed : 16179254).
Product promise
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