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AB151719

Anti-PRAS40 antibody [EPR6263(2)]

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(8 Publications)

Rabbit Recombinant Monoclonal PRAS40 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 8 publications.

View Alternative Names

PRAS40, AKT1S1, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PRAS40 with ab151719 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling PRAS40 with ab151719 at 1/10 dilution (red), compared to a nonspecific control antibody (green).

Western blot - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)
  • WB

Lab

Western blot - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)

Lanes 1 - 4 : Merged signal (red and green). Green - ab151719 observed at 27 kDa. Red - loading control, ab130007, observed at 124 kDa.

ab151719 was shown to recognize PRAS40 in wild-type HAP1 cells as signal was lost at the expected MW in AKT1S1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and AKT1S1 knockout samples were subjected to SDS-PAGE. ab151719 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PRAS40 antibody [EPR6263(2)] (ab151719) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

AKT1S1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF-7 whole cell lysate at 20 µg

Predicted band size: 27 kDa

Observed band size: 40 kDa

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Western blot - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)
  • WB

Unknown

Western blot - Anti-PRAS40 antibody [EPR6263(2)] (AB151719)

All lanes:

Western blot - Anti-PRAS40 antibody [EPR6263(2)] (ab151719) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

SHSY5Y cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 27 kDa

Observed band size: 40 kDa

false

  • Carrier free

    Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6263(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRAS40 also known as Proline-Rich AKT1 Substrate 40 kDa is a protein that serves a mechanical function as a binding partner within the mTOR complex. This protein with a molecular mass of approximately 40 kDa works within cellular environments to regulate various kinases. PRAS40 expresses ubiquitously but shows significant activity within tissues that have high metabolic roles such as muscle and fat. Its interaction with the mammalian target of rapamycin (mTOR) complex makes it an important component in cellular processes.
Biological function summary

PRAS40 acts as an inhibitory regulator in cellular growth and metabolism. PRAS40 is a part of the mTORC1 complex where it connects with other proteins to modulate the actions of mTOR a central regulator of cellular metabolism. By interacting with the mTORC1 complex PRAS40 can control protein synthesis and cell cycle progression. Furthermore PRAS40 responds to various cellular signals actively contributing to balancing growth factors and nutritional signals in cells.

Pathways

PRAS40 plays a significant role in the mTOR signaling pathway a critical pathway for cell growth and metabolism. PRAS40 regulation within this pathway involves the Akt protein which phosphorylates and modulates PRAS40 activity. Additionally PRAS40 has a connection to insulin signaling pathways where it impacts glucose uptake and energy homeostasis. Through interactions in these pathways PRAS40 balances cellular responses to growth factors and metabolic conditions.

PRAS40 shows significant implications in cancer and diabetes. Abnormal regulation of PRAS40 has links to cancer progression due to its role in controlling cell growth via mTOR pathways. Dysregulation in PRAS40 function can enhance cell proliferation and contribute to oncogenesis. Likewise PRAS40 is relevant in diabetes especially through its role in insulin signaling. Imbalances here can disrupt glucose metabolism and exacerbate diabetic conditions. Interactions with proteins such as Akt highlight its potential role in managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 17277771, PubMed : 17386266, PubMed : 17510057, PubMed : 29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site (PubMed : 29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed : 17386266, PubMed : 18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed : 16174443, PubMed : 18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity).
See full target information AKT1S1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:39 PubMed39901252

2025

Enhanced therapeutic effects of hypoxia-preconditioned mesenchymal stromal cell-derived extracellular vesicles in renal ischemic injury.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Yuan,Jie Liu,Liang Zhong,Pengtao Liu,Ting Li,Kexin Yang,Wei Gao,Guangyuan Zhang,Jie Sun,Xiangyu Zou

Journal of Cancer 15:645-658 PubMed38213722

2024

HSPB8 Facilitates the Oncogenesis and Advancement of Bladder Cancer via Activation of HSP27.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Du,Kai Guo,Peng Wang,Jianye Zhong,Ning Jiang

Scientific reports 12:2189 PubMed35140327

2022

Identification and validation of a cigarette smoke-related five-gene signature as a prognostic biomarker in kidney renal clear cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yefei Huang,Qinzhi Wang,Yu Tang,Zixuan Liu,Guixiang Sun,Zhaojun Lu,Yansu Chen

GeroScience 42:1157-1173 PubMed32578071

2020

Gene expression and regulatory factors of the mechanistic target of rapamycin (mTOR) complex 1 predict mammalian longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Mota-Martorell,Mariona Jove,Irene Pradas,Rebeca Berdún,Isabel Sanchez,Alba Naudi,Eloi Gari,Gustavo Barja,Reinald Pamplona

Frontiers in cell and developmental biology 8:429 PubMed32656205

2020

Long Noncoding RNA LINC01134 Promotes Hepatocellular Carcinoma Metastasis via Activating AKT1S1 and NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wang,Yan Chen,Kunlun Chen,Lei Zhang

Experimental and therapeutic medicine 19:3565-3570 PubMed32346419

2020

PTEN inhibitor VO-OHpic suppresses TSC2 MEFs proliferation by excessively inhibiting autophagy via the PTEN/PRAS40 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenda Wang,Xu Wang,Hao Guo,Yi Cai,Yushi Zhang,Hanzhong Li

Frontiers in endocrinology 10:797 PubMed31849836

2019

The Apoptosis Regulator 14-3-3η and Its Potential as a Therapeutic Target in Pituitary Oncocytoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sida Zhao,Bin Li,Chuzhong Li,Hua Gao,Yazhou Miao,Yue He,Hongyun Wang,Lei Gong,Dan Li,Yazhuo Zhang,Jie Feng

Cell death & disease 10:874 PubMed31745071

2019

Inhibition of PTP1B blocks pancreatic cancer progression by targeting the PKM2/AMPK/mTOC1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Xu,Ning Wu,Xiangqian Li,Chuanlong Guo,Chao Li,Bo Jiang,Huaizhi Wang,Dayong Shi
View all publications
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