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Rabbit Recombinant Monoclonal PRAS40 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

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Images

Western blot - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (AB249037), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (AB249037), expandable thumbnail
  • Western blot - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (AB249037), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PRAS40 antibody [EPR6263(2)] - BSA and Azide free (AB249037), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Tested
Mouse
Expected
Tested
Not recommended
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed:17277771, PubMed:17386266, PubMed:17510057, PubMed:29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site (PubMed:29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed:17386266, PubMed:18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed:16174443, PubMed:18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity).

Alternative names

PRAS40, AKT1S1, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

Recommended products

Rabbit Recombinant Monoclonal PRAS40 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6263(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249037 is the carrier-free version of Anti-PRAS40 antibody [EPR6263(2)] ab151719.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PRAS40 also known as Proline-Rich AKT1 Substrate 40 kDa is a protein that serves a mechanical function as a binding partner within the mTOR complex. This protein with a molecular mass of approximately 40 kDa works within cellular environments to regulate various kinases. PRAS40 expresses ubiquitously but shows significant activity within tissues that have high metabolic roles such as muscle and fat. Its interaction with the mammalian target of rapamycin (mTOR) complex makes it an important component in cellular processes.

Biological function summary

PRAS40 acts as an inhibitory regulator in cellular growth and metabolism. PRAS40 is a part of the mTORC1 complex where it connects with other proteins to modulate the actions of mTOR a central regulator of cellular metabolism. By interacting with the mTORC1 complex PRAS40 can control protein synthesis and cell cycle progression. Furthermore PRAS40 responds to various cellular signals actively contributing to balancing growth factors and nutritional signals in cells.

Pathways

PRAS40 plays a significant role in the mTOR signaling pathway a critical pathway for cell growth and metabolism. PRAS40 regulation within this pathway involves the Akt protein which phosphorylates and modulates PRAS40 activity. Additionally PRAS40 has a connection to insulin signaling pathways where it impacts glucose uptake and energy homeostasis. Through interactions in these pathways PRAS40 balances cellular responses to growth factors and metabolic conditions.

Associated diseases and disorders

PRAS40 shows significant implications in cancer and diabetes. Abnormal regulation of PRAS40 has links to cancer progression due to its role in controlling cell growth via mTOR pathways. Dysregulation in PRAS40 function can enhance cell proliferation and contribute to oncogenesis. Likewise PRAS40 is relevant in diabetes especially through its role in insulin signaling. Imbalances here can disrupt glucose metabolism and exacerbate diabetic conditions. Interactions with proteins such as Akt highlight its potential role in managing these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com