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AB248564

Anti-PRAS40 antibody [EPR6402] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal PRAS40 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

PRAS40, AKT1S1, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

4 Images
Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)
  • WB

Lab

Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)

This data was developed using ab133584, the same antibody clone in a different buffer formulation.

Lanes 1 - 4 : Merged signal (red and green). Green - ab133584 observed at 40 kDa. Red - loading control, ab130007, observed at 125 kDa.

ab133584 was shown to specifically react with PRAS40 in wild-type HAP1 cells as signal was lost in AKT1S1 (PRAS40) knockout cells. Wild-type and AKT1S1 (PRAS40) knockout samples were subjected to SDS-PAGE. ab133584 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PRAS40 antibody [EPR6402] (<a href='/en-us/products/primary-antibodies/pras40-antibody-epr6402-ab133584'>ab133584</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

AKT1S1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF-7 whole cell lysate at 20 µg

Predicted band size: 27 kDa

Observed band size: 40 kDa

false

Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)
  • WB

Lab

Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133584).

Lanes 1 - 4 : Merged signal (red and green). Green - ab133584 observed at 40 kDa. Red - loading control, ab130007, observed at 125 kDa.

ab133584 was shown to specifically react with PRAS40 in wild-type HAP1 cells as signal was lost in AKT1S1 (PRAS40) knockout cells. Wild-type and AKT1S1 (PRAS40) knockout samples were subjected to SDS-PAGE. ab133584 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (ab248564) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

AKT1S1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

MCF-7 whole cell lysate at 20 µg

Predicted band size: 27 kDa

Observed band size: 40 kDa

false

Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)
  • WB

Unknown

Western blot - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)

This data was developed using ab133584, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PRAS40 antibody [EPR6402] (<a href='/en-us/products/primary-antibodies/pras40-antibody-epr6402-ab133584'>ab133584</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

L6 cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

PC12 cell lysate at 10 µg

Lane 6:

NIH 3T3 cell lysate at 10 µg

Lane 7:

293T cell lysate at 10 µg

Lane 8:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 27 kDa

false

OI-RD Scanning - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PRAS40 antibody [EPR6402] - BSA and Azide free (AB248564)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6402

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab248564 is the carrier-free version of ab133584.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRAS40 also known as Proline-Rich AKT1 Substrate 40 kDa is a protein that serves a mechanical function as a binding partner within the mTOR complex. This protein with a molecular mass of approximately 40 kDa works within cellular environments to regulate various kinases. PRAS40 expresses ubiquitously but shows significant activity within tissues that have high metabolic roles such as muscle and fat. Its interaction with the mammalian target of rapamycin (mTOR) complex makes it an important component in cellular processes.
Biological function summary

PRAS40 acts as an inhibitory regulator in cellular growth and metabolism. PRAS40 is a part of the mTORC1 complex where it connects with other proteins to modulate the actions of mTOR a central regulator of cellular metabolism. By interacting with the mTORC1 complex PRAS40 can control protein synthesis and cell cycle progression. Furthermore PRAS40 responds to various cellular signals actively contributing to balancing growth factors and nutritional signals in cells.

Pathways

PRAS40 plays a significant role in the mTOR signaling pathway a critical pathway for cell growth and metabolism. PRAS40 regulation within this pathway involves the Akt protein which phosphorylates and modulates PRAS40 activity. Additionally PRAS40 has a connection to insulin signaling pathways where it impacts glucose uptake and energy homeostasis. Through interactions in these pathways PRAS40 balances cellular responses to growth factors and metabolic conditions.

PRAS40 shows significant implications in cancer and diabetes. Abnormal regulation of PRAS40 has links to cancer progression due to its role in controlling cell growth via mTOR pathways. Dysregulation in PRAS40 function can enhance cell proliferation and contribute to oncogenesis. Likewise PRAS40 is relevant in diabetes especially through its role in insulin signaling. Imbalances here can disrupt glucose metabolism and exacerbate diabetic conditions. Interactions with proteins such as Akt highlight its potential role in managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 17277771, PubMed : 17386266, PubMed : 17510057, PubMed : 29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site (PubMed : 29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed : 17386266, PubMed : 18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed : 16174443, PubMed : 18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity).
See full target information AKT1S1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Psychiatry investigation 22:699-713 PubMed40566894

2025

Transcriptional Landscape and Biomarker Discovery for Endoplasmic Reticulum Stress in Alzheimer's Disease: An Ex Vivo Study Using Patients-Derived Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yeojin Kim,You Jin Nam,Sunwoo Yoon,Young Joon Cho,Ho Min Song,Seongmin Kim,Donghyuk Shin,Jin Young Noh,Sun Min Lee,So Young Moon,Eun-Joo Kim,Soo Hyun Cho,Byeong C Kim,Seong Hye Choi,Sang Won Seo,Jin Wook Choi,Young-Sil An,Bumhee Park,Young Joon Park,Hee Young Kang,Hyun Goo Woo,Yong Hyuk Cho,Sunhwa Hong,Sang Joon Son,Sang-Rae Lee,Chang Hyung Hong,Hyun Woong Roh
View all publications
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Product promise

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