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AB226994

Anti-PRAS40 (phospho S183) antibody

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(2 Publications)

Rabbit Polyclonal PRAS40 phospho S183 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human AKT1S1 phospho S183.

View Alternative Names

PRAS40, AKT1S1, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

1 Images
Western blot - Anti-PRAS40 (phospho S183) antibody (AB226994)
  • WB

Supplier Data

Western blot - Anti-PRAS40 (phospho S183) antibody (AB226994)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-PRAS40 (phospho S183) antibody (ab226994) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), starved for 16 hours, whole cell extract at 40 µg

Lane 2:

HEK-293T, starved for 16 hours, and treated with 100 nM Rapamycin treated for 30 minutes whole cell extract at 40 µg

Lane 3:

HEK-293T, starved for 16 hours and treated with 100 nM insulin for 30 minutes, whole cell extract at 40 µg

Lane 4:

HEK-293T, starved for 16 hours and treated with 100 nM Rapamycin and 100 nM insulin for 30 minutes, whole cell extract at 40 µg

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human AKT1S1 phospho S183. The exact immunogen used to generate this antibody is proprietary information.

Q96B36

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRAS40 also known as Proline-Rich AKT1 Substrate 40 kDa is a protein that serves a mechanical function as a binding partner within the mTOR complex. This protein with a molecular mass of approximately 40 kDa works within cellular environments to regulate various kinases. PRAS40 expresses ubiquitously but shows significant activity within tissues that have high metabolic roles such as muscle and fat. Its interaction with the mammalian target of rapamycin (mTOR) complex makes it an important component in cellular processes.
Biological function summary

PRAS40 acts as an inhibitory regulator in cellular growth and metabolism. PRAS40 is a part of the mTORC1 complex where it connects with other proteins to modulate the actions of mTOR a central regulator of cellular metabolism. By interacting with the mTORC1 complex PRAS40 can control protein synthesis and cell cycle progression. Furthermore PRAS40 responds to various cellular signals actively contributing to balancing growth factors and nutritional signals in cells.

Pathways

PRAS40 plays a significant role in the mTOR signaling pathway a critical pathway for cell growth and metabolism. PRAS40 regulation within this pathway involves the Akt protein which phosphorylates and modulates PRAS40 activity. Additionally PRAS40 has a connection to insulin signaling pathways where it impacts glucose uptake and energy homeostasis. Through interactions in these pathways PRAS40 balances cellular responses to growth factors and metabolic conditions.

PRAS40 shows significant implications in cancer and diabetes. Abnormal regulation of PRAS40 has links to cancer progression due to its role in controlling cell growth via mTOR pathways. Dysregulation in PRAS40 function can enhance cell proliferation and contribute to oncogenesis. Likewise PRAS40 is relevant in diabetes especially through its role in insulin signaling. Imbalances here can disrupt glucose metabolism and exacerbate diabetic conditions. Interactions with proteins such as Akt highlight its potential role in managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 17277771, PubMed : 17386266, PubMed : 17510057, PubMed : 29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site (PubMed : 29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed : 17386266, PubMed : 18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed : 16174443, PubMed : 18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity).
See full target information AKT1S1 phospho S183
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Acta biochimica et biophysica Sinica 57:712-726 PubMed39578715

2024

PDK1 promotes epithelial ovarian cancer progression by upregulating BGN.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Lina Yan,Xin Fu,Ziqi Tao,Shuna Liu,Rong Li,Ting Wang,Yepeng Mao,Wenwen Shang,Mi Gong,Xuemei Jia,Fang Wang

Frontiers in cell and developmental biology 8:429 PubMed32656205

2020

Long Noncoding RNA LINC01134 Promotes Hepatocellular Carcinoma Metastasis via Activating AKT1S1 and NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wang,Yan Chen,Kunlun Chen,Lei Zhang
View all publications
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