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AB134084

Anti-PRAS40 (phospho T246) antibody [EPR6262-107]

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(5 Publications)

Rabbit Recombinant Monoclonal PRAS40 phospho T246 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

PRAS40, AKT1S1, Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate

1 Images
Western blot - Anti-PRAS40 (phospho T246) antibody [EPR6262-107] (AB134084)
  • WB

Unknown

Western blot - Anti-PRAS40 (phospho T246) antibody [EPR6262-107] (AB134084)

All lanes:

Western blot - Anti-PRAS40 (phospho T246) antibody [EPR6262-107] (ab134084) at 1/1000 dilution

Lane 1:

HeLa cell lysate, untreated at 10 µg

Lane 2:

HeLa cell lysate, treated with Lambda Phosphatase at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa

false

  • Carrier free

    Anti-PRAS40 (phospho T246) antibody [EPR6262-107] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6262-107

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRAS40 also known as Proline-Rich AKT1 Substrate 40 kDa is a protein that serves a mechanical function as a binding partner within the mTOR complex. This protein with a molecular mass of approximately 40 kDa works within cellular environments to regulate various kinases. PRAS40 expresses ubiquitously but shows significant activity within tissues that have high metabolic roles such as muscle and fat. Its interaction with the mammalian target of rapamycin (mTOR) complex makes it an important component in cellular processes.
Biological function summary

PRAS40 acts as an inhibitory regulator in cellular growth and metabolism. PRAS40 is a part of the mTORC1 complex where it connects with other proteins to modulate the actions of mTOR a central regulator of cellular metabolism. By interacting with the mTORC1 complex PRAS40 can control protein synthesis and cell cycle progression. Furthermore PRAS40 responds to various cellular signals actively contributing to balancing growth factors and nutritional signals in cells.

Pathways

PRAS40 plays a significant role in the mTOR signaling pathway a critical pathway for cell growth and metabolism. PRAS40 regulation within this pathway involves the Akt protein which phosphorylates and modulates PRAS40 activity. Additionally PRAS40 has a connection to insulin signaling pathways where it impacts glucose uptake and energy homeostasis. Through interactions in these pathways PRAS40 balances cellular responses to growth factors and metabolic conditions.

PRAS40 shows significant implications in cancer and diabetes. Abnormal regulation of PRAS40 has links to cancer progression due to its role in controlling cell growth via mTOR pathways. Dysregulation in PRAS40 function can enhance cell proliferation and contribute to oncogenesis. Likewise PRAS40 is relevant in diabetes especially through its role in insulin signaling. Imbalances here can disrupt glucose metabolism and exacerbate diabetic conditions. Interactions with proteins such as Akt highlight its potential role in managing these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), an evolutionarily conserved central nutrient sensor that stimulates anabolic reactions and macromolecule biosynthesis to promote cellular biomass generation and growth (PubMed : 17277771, PubMed : 17386266, PubMed : 17510057, PubMed : 29236692). In absence of insulin and nutrients, AKT1S1 associates with the mTORC1 complex and directly inhibits mTORC1 activity by blocking the MTOR substrate-recruitment site (PubMed : 29236692). In response to insulin and nutrients, AKT1S1 dissociates from mTORC1 (PubMed : 17386266, PubMed : 18372248). Its activity is dependent on its phosphorylation state and binding to 14-3-3 (PubMed : 16174443, PubMed : 18372248). May also play a role in nerve growth factor-mediated neuroprotection (By similarity).
See full target information AKT1S1 pT246
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:645-658 PubMed38213722

2024

HSPB8 Facilitates the Oncogenesis and Advancement of Bladder Cancer via Activation of HSP27.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Du,Kai Guo,Peng Wang,Jianye Zhong,Ning Jiang

GeroScience 42:1157-1173 PubMed32578071

2020

Gene expression and regulatory factors of the mechanistic target of rapamycin (mTOR) complex 1 predict mammalian longevity.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Mota-Martorell,Mariona Jove,Irene Pradas,Rebeca Berdún,Isabel Sanchez,Alba Naudi,Eloi Gari,Gustavo Barja,Reinald Pamplona

Experimental and therapeutic medicine 19:3565-3570 PubMed32346419

2020

PTEN inhibitor VO-OHpic suppresses TSC2 MEFs proliferation by excessively inhibiting autophagy via the PTEN/PRAS40 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenda Wang,Xu Wang,Hao Guo,Yi Cai,Yushi Zhang,Hanzhong Li

Scientific reports 10:2140 PubMed32034177

2020

Assembly and Folding Properties of Cytosolic IgG Intrabodies.

Applications

Unspecified application

Species

Unspecified reactive species

Youngsil Seo,Yeonjin Lee,Minjae Kim,Hyunjoon Park,Myung-Hee Kwon

Frontiers in endocrinology 10:797 PubMed31849836

2019

The Apoptosis Regulator 14-3-3η and Its Potential as a Therapeutic Target in Pituitary Oncocytoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sida Zhao,Bin Li,Chuzhong Li,Hua Gao,Yazhou Miao,Yue He,Hongyun Wang,Lei Gong,Dan Li,Yazhuo Zhang,Jie Feng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com